Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.
Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.
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胞质分裂结的分子结构预示着收缩环的收缩率。
DOI：
10.1083/jcb.202008032
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发表时间：
2021-03-01
期刊：
影响因子：
--
通讯作者：
Laplante C
中科院分区：
文献类型：
--
作者：
Bellingham-Johnstun K;Anders EC;Ravi J;Bruinsma C;Laplante C
收缩环在胞质分裂过程中的功能取决于其分子结构。本研究使用活裂殖酵母细胞中的单分子定位显微镜来显示肌球蛋白-II Myo 2 p的不同分子组织与收缩环的不同收缩率相关。胞质分裂蛋白质的分子结构控制收缩环的功能。我们用单分子定位显微镜在活细胞中阐明胞质分裂蛋白的分子组织，并将其与收缩环的收缩率。野生型裂殖酵母细胞通过称为节点的皮质蛋白复合物的聚结组装收缩环，而没有Anillin/Mid 1 p（Δ mid 1）的细胞缺乏可见的节点，但组装收缩环，能够从链的成环中收缩。我们利用Δ mid 1收缩环组装机制来确定两种不同的分子组织（节点与链）如何产生功能性收缩环。与先前的解释相反，节点聚集在Δ mid 1细胞中。我们的研究结果表明，Myo 2 p头凝聚后与肌动蛋白丝的相互作用和多余的Myo 2 p头绑定到肌动蛋白丝阻碍收缩，从而降低收缩率。我们的工作建立了一个预测节点的分子组织和收缩环的行为之间的相关性。
The function of the contractile ring during cytokinesis depends on its molecular organization. This study uses single-molecule localization microscopy in live fission yeast cells to show that distinct molecular organizations of the myosin-II Myo2p correlate with different constriction rates of the contractile ring. The molecular organization of cytokinesis proteins governs contractile ring function. We used single molecule localization microscopy in live cells to elucidate the molecular organization of cytokinesis proteins and relate it to the constriction rate of the contractile ring. Wild-type fission yeast cells assemble contractile rings by the coalescence of cortical proteins complexes called nodes whereas cells without Anillin/Mid1p (Δmid1) lack visible nodes yet assemble contractile rings competent for constriction from the looping of strands. We leveraged the Δmid1 contractile ring assembly mechanism to determine how two distinct molecular organizations, nodes versus strands, can yield functional contractile rings. Contrary to previous interpretations, nodes assemble in Δmid1 cells. Our results suggest that Myo2p heads condense upon interaction with actin filaments and an excess number of Myo2p heads bound to actin filaments hinders constriction thus reducing the constriction rate. Our work establishes a predictive correlation between the molecular organization of nodes and the behavior of the contractile ring.
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影响因子：
7.8
作者：
Coffman, Valerie C.;Sees, Jennifer A.;Wu, Jian-Qiu
通讯作者：
Wu, Jian-Qiu
DOI：
10.1083/jcb.143.6.1603
发表时间：
1998-12-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bähler J;Steever AB;Wheatley S;Wang Yl;Pringle JR;Gould KL;McCollum D
通讯作者：
McCollum D
影响因子：
64.8
作者：
Moseley, James B.;Mayeux, Adeline;Nurse, Paul
通讯作者：
Nurse, Paul
DOI：
10.1083/jcb.200402097
发表时间：
2004-06-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Motegi F;Mishra M;Balasubramanian MK;Mabuchi I
通讯作者：
Mabuchi I
影响因子：
3.3
作者：
Coffman, Valerie C.;Nile, Aaron H.;Wu, Jian-Qiu
通讯作者：
Wu, Jian-Qiu