Long-term effect of green tea extract during lactation on AMPK expression in rat offspring exposed to fetal malnutrition.
Long-term effect of green tea extract during lactation on AMPK expression in rat offspring exposed to fetal malnutrition.
复制标题
哺乳期绿茶提取物对胎儿营养不良大鼠后代 AMPK 表达的长期影响。
DOI：
10.1016/j.nut.2013.03.021
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发表时间：
2013
期刊：
影响因子：
4.4
通讯作者：
Kurasaki M.
中科院分区：
文献类型：
--
作者：
Sato S;Mukai Y;Hamaya M;Sun Y;Kurasaki M.
胎儿和新生儿的环境是成年期疾病风险的重要决定因素。本研究的目的是确定是否产妇绿色茶提取物（GTE）的摄入量在哺乳期影响腺苷单磷酸活化蛋白激酶（AMPK）的表达和活性的蛋白质限制的dams在gestation.MethodsPregnant Wistar大鼠的雄性后代的肾脏喂养控制（C）或低蛋白饮食（LP）在妊娠期间。分娩后，母鼠在哺乳期接受对照或含GTE的对照饲料，如下所示：C对照饲料（CC）、LP对照饲料（LPC）、LP含0.12% GTE的对照饲料（LPL）或LP含0.24% GTE的对照饲料（LPH）。在第3周处死每只母鼠的一些雄性幼仔，其余雄性幼仔饲喂标准饲料并在第30周处死。结果LPH组第3周AMPKα磷酸化水平明显高于LPC组（P < 0. 05），LPH组第3周AMPKα磷酸化水平明显高于LPC组（P < 0. 05）。30周时，LPL和LPH组总AMPK和磷酸化AMPK蛋白水平低于LPC组。在LPL和LPH组的mTOR和Akt在第30周的蛋白水平低于那些在LPC group.ConclusionGTE摄入量在哺乳期调节AMPK，Akt，和mTOR的表达在肾脏的成年男性后代的母鼠喂蛋白质限制饮食，并可能导致长期的改变，这些蛋白质在肾脏的表达。
ObjectiveThe fetal and neonatal environments are important determinants of disease risk in adult life. The aim of this study was to determine whether maternal green tea extract (GTE) intake during lactation affects the expression and activity of adenosine monophosphate-activated protein kinase (AMPK) in the kidneys of male offspring of protein-restricted dams during gestation.MethodsPregnant Wistar rats were fed control (C) or low-protein diets (LP) during gestation. Following delivery, dams received a control or GTE-containing control diet during lactation as follows: C on control diet (CC), LP on control diet (LPC), LP on 0.12% GTE-containing control diet (LPL), or LP on 0.24% GTE-containing control diet (LPH). Some of the male pups from each dam were sacrificed at week 3, and the remaining male pups were fed a standard diet and sacrificed at week 30. Blood chemistry and expression levels of AMPKα, mammalian target of rapamycin (mTOR), and Akt in the kidneys of the male offspring were examined.ResultsThe level of phosphorylated AMPKα in the LPH group at week 3 was higher than that in the LPC group. At week 30, the protein levels of total and phosphorylated AMPK in the LPL and LPH groups were lower than those in the LPC group. The protein levels of mTOR and Akt at week 30 in the LPL and LPH groups were lower than those in the LPC group.ConclusionGTE intake during lactation modulates AMPK, Akt, and mTOR expression in the kidneys of the adult male offspring of dams fed a protein-restricted diet and may induce long-term alterations in the expressions of these proteins in the kidneys.