Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages
Isolation, Culture, and Polarization of Murine Bone Marrow-Derived and Peritoneal Macrophages
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DOI：
10.1007/978-1-4939-2929-0_6
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Pello, Oscar M.
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文献类型：
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作者：
Pineda-Torra, Ines;Gage, Matthew;Pello, Oscar M.
巨噬细胞是最特殊的吞噬细胞，在各种外部刺激下获得特定的表型和功能。培养小鼠骨髓或腹膜巨噬细胞是研究巨噬细胞表型和功能对特定刺激的反应的一种非常强大的技术，尽可能类似于在各种病理生理环境中观察到的条件。本章概述了分离和培养小鼠骨髓和腹膜巨噬细胞的方法。此外，我们描述了这些巨噬细胞如何被“极化”以获得与动脉粥样硬化特别相关的特定巨噬细胞亚群。
Macrophages are the most specialized phagocytic cells, and acquire specific phenotypes and functions in response to a variety of external triggers. Culture of bone marrow-derived or peritoneal macrophages from mice represents an exceptionally powerful technique to investigate macrophage phenotypes and functions in response to specific stimuli, resembling as much as possible the conditions observed in various pathophysiological settings. This chapter outlines protocols used to isolate and culture murine bone marrow-derived and peritoneal macrophages. Furthermore, we describe how these macrophages can be "polarized" to obtain specific macrophage subsets with special relevance to atherosclerosis.