Cellular localization of guanylin and uroguanylin mRNAs in human and rat duodenal and colonic mucosa.
Cellular localization of guanylin and uroguanylin mRNAs in human and rat duodenal and colonic mucosa.
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DOI：
10.1007/s00441-016-2393-y
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发表时间：
2016-08
影响因子：
3.6
通讯作者：
Gustafsson BI
中科院分区：
文献类型：
--
作者：
Brenna Ø;Furnes MW;Munkvold B;Kidd M;Sandvik AK;Gustafsson BI
鸟苷素(GUCA2a/Guca2a/GN)和尿鸟苷(GUCA2b/Guca2b/UGN)在胃肠道表达，与离子和液体动态平衡、饱腹感、腹痛、生长和肠道屏障完整性有关。它们的细胞来源存在争议，包括杯状细胞、肠/结肠细胞、肠内分泌(EE)细胞和簇状细胞。因此，我们用原位杂交(ISH)研究了人和大鼠十二指肠和结肠上皮中GN和UGN mRNAs的细胞来源，以确定与嗜铬粒蛋白A(CHGA/CHGA/CGA；肠嗜铬细胞[EC]细胞)、防御素α6(DEFA6/Defa6；Paneth细胞)、粘蛋白2(MUC2/Muc2；杯状细胞)和部分簇状细胞标志物的共表达。GUCA2a/Guca2a在人和大鼠结肠中的表达定位于杯状细胞和结肠细胞。在人十二指肠组织中，GUCA2A在Paneth细胞中表达，在绒毛上皮细胞中表达较少。在大鼠十二指肠中，Guca2a仅定位于杯状细胞。Guca2b在大鼠结肠局灶性表达。在人、大鼠十二指肠和人结肠中，GUCA2B/GUCA2b表达于散在的孤立上皮细胞中，有的呈簇状细胞样。在人十二指肠细胞中，GUCA2A和GUCA2B均未与CHGA共表达。因此，EC细胞可能不是人类GN或UGN的主要来源，但其他EE细胞作为GN或UGN的来源也不完全被排除在外。没有发现与簇状细胞标记有令人信服的重叠。我们首次证实了GUCA2B在人十二指肠中的细胞表达。GN和UGN在十二指肠和结肠以及人和大鼠肠道中的特异性细胞分布不同。本文的在线版本(doi：10.1007/s00441-0162393-y)包含补充材料，可供授权用户使用。
Guanylin (GUCA2A/Guca2a/GN) and uroguanylin (GUCA2B/Guca2b/UGN) are expressed in the gastrointestinal tract and have been implicated in ion and fluid homeostasis, satiety, abdominal pain, growth and intestinal barrier integrity. Their cellular sources are debated and include goblet cells, entero-/colonocytes, enteroendocrine (EE) cells and tuft cells. We therefore investigated the cellular sources of GN and UGN mRNAs in human and rat duodenal and colonic epithelium with in situ hybridization (ISH) to determine co-expression with Chromogranin A (CHGA/Chga/CgA; enterochromaffin [EC] cells), defensin alpha 6 (DEFA6/Defa6; Paneth cells), mucin 2 (MUC2/Muc2; goblet cells) and selected tuft cell markers. GUCA2A/Guca2a expression was localized to goblet cells and colonocytes in human and rat colon. In human duodenum, GUCA2A was expressed in Paneth cells and was scarce in villous epithelial cells. In rat duodenum, Guca2a was only localized to goblet cells. Guca2b was focally expressed in rat colon. In human and rat duodenum and in human colon, GUCA2B/Guca2b was expressed in dispersed solitary epithelial cells, some with a tuft cell-like appearance. Neither GUCA2A nor GUCA2B were co-expressed with CHGA in human duodenal cells. Consequently, EC cells are probably not the major source of human GN or UGN but other EE cells as a source of GN or UGN are not entirely excluded. No convincing overlap with tuft cell markers was found. For the first time, we demonstrate the cellular expression of GUCA2B in human duodenum. The specific cellular distribution of both GN and UGN differs between duodenum and colon and between human and rat intestines. The online version of this article (doi:10.1007/s00441-016-2393-y) contains supplementary material, which is available to authorized users.