Oxidative stress by visible light irradiation suppresses immunoglobulin production in mouse spleen lymphocytes
Oxidative stress by visible light irradiation suppresses immunoglobulin production in mouse spleen lymphocytes
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DOI：
10.1271/bbb.65.593
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发表时间：
2001-03-01
影响因子：
1.6
通讯作者：
Yamada, K
中科院分区：
文献类型：
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作者：
Miyazaki, Y;Yamasaki, M;Yamada, K
本研究采用可见光照射诱导小鼠脾淋巴细胞氧化应激，观察脂质过氧化对免疫球蛋白（IG）产生的影响。当细胞培养72小时时，培养上清液中的IG含量随着照射时间的延长而逐渐降低，细胞活力也随着照射时间的延长而降低。细胞内的磷脂酰胆碱氢过氧化物（PCOH）水平和硫代巴比妥酸反应物质（TBARS）的培养上清液中的值作为脂质过氧化的指标，我们发现，小鼠脾淋巴细胞产生IG抑制伴随着细胞内磷脂的过氧化反应的进展。细胞膜流动性也明显降低，但细胞内IG水平没有改变。这些结果表明，细胞内脂质过氧化是通过降低细胞活力和抑制IG合成和分泌来抑制小鼠脾淋巴细胞产生IG的原因。
In this study,we attempted to induce the oxidative stress in mouse spleen lymphocytes with visible light irradiation and examined the effects of lipid peroxidation on immunoglobulin (Ig) production, The spleen lymphocytes were isolated from 8-week-old male balb/c mice and irradiated with 300 W visible light. When the cells were cultured for 72 hr, Ig contents in culture supernatants were decreased gradually by irradiation for over 30 min. The cell viability was also lowered by the irradiation. Intracellular phosphatidylcholine hydroperoxide (PCOOH) levels and thiobarbituric acid-reactive substances (TBARs) values in culture supernatants were measured as indices of lipid peroxidation and we found that Ig production by mouse spleen lymphocytes was suppressed accompanied with the progress of peroxidation of intracellular phospholipids. Cell membrane fluidity was also significantly decreased, but the intracellular Ig level was not changed in the irradiated cells. These results suggest that the peroxidation of intracellular lipids is a cause of the suppression of Ig production by mouse spleen lymphocytes via lowering cell viability and suppressing Ig synthesis and secretion.