Pentraxin3 and high-sensitive C-reactive protein are independent inflammatory markers released during high-intensity exercise
Pentraxin3 and high-sensitive C-reactive protein are independent inflammatory markers released during high-intensity exercise
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DOI：
10.1007/s00421-010-1572-x
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发表时间：
2010-11-01
影响因子：
3
通讯作者：
Nagai, Ryozo
中科院分区：
文献类型：
--
作者：
Nakajima, Toshiaki;Kurano, Miwa;Nagai, Ryozo
高强度运动与炎症性疾病的急性期反应有相似之处。我们研究了急性运动对炎症标志物、血浆正五聚蛋白3（PTX 3）和血清高敏C反应蛋白（CRP）（hsCRP）的影响。9名健康男性受试者（41 +/- 3岁）参加了研究。每名受试者进行三种类型的运动;以无氧阈值（AT）的70%工作负荷进行30分钟的测力计运动（70%AT运动）、峰值测力计运动（峰值EX，20瓦增加/分钟直到疲劳）和70%1 RM的阻力运动（70%RE）直到耗尽。我们测量了血浆PTX 3、血清hsCRP、乳酸盐、去甲肾上腺素（NOR）、白色血细胞（WBC）、白细胞介素-6（IL-6）和髓过氧化物酶（MPO）（中性粒细胞脱粒的标志物）。采用流式细胞仪检测运动对中性粒细胞内PTX 3和MPO的影响。在70%RE和峰值EX后，循环PTX 3和hsCRP立即显著升高，而在70%AT运动后，它们没有升高。运动诱导的PTX 3和hsCRP相对于静息水平的增加倍数与WBC、NOR、乳酸和MPO的变化呈正相关。运动诱导的IL-6增加倍数与NOR呈正相关，但与PTX 3和hsCRP无相关性。70%RE运动后立即分离的中性粒细胞，而不是70%AT运动后立即分离的中性粒细胞，其PTX 3和MPO的平均荧光比运动前血液中的平均荧光低。这些结果提供了证据表明，高强度运动显着增加循环TX 3和hsCRP。提示外周血中性粒细胞的释放参与运动诱导的血浆PTX 3升高。
High-intensity exercise shares similarities with acute phase responses of inflammatory diseases. We investigated the influences of acute exercise on inflammatory markers, plasma pentraxin3 (PTX3) and serum high-sensitive C-reactive protein (CRP) (hsCRP). Nine healthy male subjects (41 +/- 3 years old) participated. Each subject performed three types of exercise; ergometer exercise at 70% workload of anaerobic threshold (AT) for 30 min (70% AT exercise), peak ergometer exercise (peak EX, 20 watt increase/min until fatigue) and resistance exercises of 70% 1 RM (70% RE) until exhaustion. We measured plasma PTX3, serum hsCRP, lactate, noradrenaline (NOR), white blood cells (WBC), interleukin-6 (IL-6) and myeloperoxidase (MPO), a marker of neutrophil degranulation. The effects of exercise on intracellular PTX3 and MPO in neutrophils were also investigated, by using flow cytometry analysis. Circulating PTX3 and hsCRP significantly increased immediately after 70% RE and peak EX, while they did not increase after 70% AT exercise. The exercise-induced fold increase in PTX3 and hsCRP relative to the resting level was positively correlated with the changes in WBC, NOR, lactate and MPO. The exercise-induced fold increase in IL-6 was positively correlated with that in NOR, but not with that in PTX3 and hsCRP. Neutrophils isolated immediately after 70% RE, but not 70% AT exercise, exhibited lower mean fluorescence for PTX3 and MPO than those from pre-exercise blood. These results provide the evidence that high-intensity exercises significantly increase circulatory PTX3 as well as hsCRP. The release from peripheral neutrophils is suggested to be involved in the exercise-induced plasma PTX3 increase.