Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging
Effective Transplantation of 2D and 3D Cultured Hepatocyte Spheroids Confirmed by Quantum Dot Imaging
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DOI：
10.1002/adbi.201800137
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发表时间：
2018-08-01
影响因子：
4.1
通讯作者：
Matsunaga, Tamihide
中科院分区：
文献类型：
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作者：
Okumura, Hiroki;Nanizawa, Eri;Matsunaga, Tamihide
肝移植是治疗终末期肝病的最有效方法，但由于供者数量有限，使用球体的肝细胞移植有望成为一种替代策略。然而，移植途径和球体大小对细胞动力学和肝细胞器官特异性积聚的影响还没有很好的表征。本文研究了尾静脉、门静脉和脾三种途径和三种球体大小(直径分别为50、100和150微米)对量子点标记的人胎肝细胞在受体小鼠肝脏和其他器官(心、肺、肾、脾)蓄积的影响。体外荧光成像显示，与其他途径相比，经门静脉移植的移植细胞在肝脏中的蓄积量最高。球体的累积效率与球体的大小有关，直径为50微米的球体比其他大小的球体具有更大的累积能力。结论：采用直径为50微米的小球体经门静脉途径进行肝细胞移植，具有较好的临床应用价值。
Liver transplantation is the most effective treatment for patients with end-stage liver diseases, but hepatocyte transplantation using spheroids is expected to become an alternative strategy owing to the limited number of liver donors. However, the effects of the transplantation route and spheroid size on cell dynamics and the organ-specific accumulation of hepatocytes are not well characterized. In this study, the influence of three delivery routes (tail vein, portal vein, and spleen) and three spheroid sizes (50, 100, and 150 mu m in diameter) on the accumulation of human fetal hepatocytes labeled with quantum dots in the liver and other organs (heart, lung, kidney, and spleen) of recipient mice is investigated. Ex vivo fluorescence imaging reveals that the accumulation of transplanted cells in the liver is highest for transplantation via the portal vein compared with other routes. The accumulation efficiency depends on spheroid size, and spheroids with a diameter of 50 mu m display greater accumulation than that of spheroids of other sizes. It is concluded that hepatocyte transplantation via the portal vein using small spheroids of 50 mu m in diameter may be effective for clinical applications.