Nuclear Overhauser effect in specifically deuterated macromolecules: NMR assay for unusual base pairing in transfer RNA.
Nuclear Overhauser effect in specifically deuterated macromolecules: NMR assay for unusual base pairing in transfer RNA.
复制标题
特定氘化大分子中的核奥弗豪瑟效应：转移 RNA 中异常碱基配对的 NMR 测定。
DOI：
10.1073/pnas.77.10.5659
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发表时间：
1980
影响因子：
11.1
通讯作者：
Tropp,J
中科院分区：
文献类型：
--
作者：
Sánchez,V;Redfield,AG;Johnston,PD;Tropp,J
我们展示了一个相当普遍的方法，用于识别从微生物中提取的大分子的NMR吸收线，基于核Overhauser效应（NOE）。在分辨的亚氨基质子共振和环碳共振之间可以观察到tRNA中的几个NOE，这些共振是腺嘌呤的C（2）质子或腺嘌呤或鸟嘌呤的C（8）质子。酵母tRNAPhe通过在2H2O中加热和生物合成在嘌呤C（8）位置被氘代。标准品A的亚氨基质子和腺嘌呤C（2）质子之间的NOE。U碱基对不会受到这种标签的影响，但其他一些可能相似的NOE，如反向Hoogsteen碱基对，应该消失。6个NOE来自标准品A。U对不消失。从甲基共振到芳香族质子共振的四个NOE消失。结果不同意以前的分配的基础上环电流理论的亚氨基质子NMR位移。
We demonstrate a fairly general method for identification of NMR absorption lines of macromolecues extracted from microorganisms, based on nuclear Overhauser effects (NOE). Several NOE in tRNA are observable between resolved imino proton resonances and ring carbon resonances that are either C(2) protons of adenine or C(8) protons of adenine or guanine. Yeast tRNAPhe was deuterated at the purine C(8) positins by heating in 2H2O and also biosynthetically. NOE between imino protons and adenine C(2) protons of standard A . U base pairs would not be affected by such a label, but some other NOE that might be otherwise similar, such as those of reverse Hoogsteen base pairs, should disappear. Six NOE were shown to be from standard A . U pairs by their nondisappearance. Four NOE from methyl resonances to aromatic proton resonances did disappear. The results disagree with previous assignments based on ring-current theories of imino proton NMR shifts.