MicroRNA-125b expression in gastric adenocarcinoma and its effect on the proliferation of gastric cancer cells.
MicroRNA-125b expression in gastric adenocarcinoma and its effect on the proliferation of gastric cancer cells.
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DOI：
10.3892/mmr.2012.1156
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发表时间：
2013
影响因子：
3.4
通讯作者：
Zhao-xu Yang;Cheng-Yi Lu;Yan-ling Yang;K. Dou;Kai-shan Tao
中科院分区：
文献类型：
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作者：
Zhao-xu Yang;Cheng-Yi Lu;Yan-ling Yang;K. Dou;Kai-shan Tao
microRNA对许多基因发挥调节作用，从而促进生理和病理过程。microRNA在肿瘤发生中的作用越来越清楚。在本研究中，我们研究了先前与前列腺癌和乳腺癌有关的microRNA-125b（miR-125b）在胃癌中的作用，特别是关于胃癌细胞的增殖和凋亡。采用实时荧光定量PCR法检测50例胃癌组织及相应癌旁组织中miR-125 b的表达。胃癌组织中miR-125 b的表达水平显著高于癌旁正常组织（P <0.05）。为了开始了解miR-125 b的表达增加如何促进胃癌，将miR-125 b模拟物转染到胃癌细胞系HGC-27中，通过流式细胞术测定增殖（CCK 8）和凋亡（Annexin V）。结果表明，与未处理和scramble处理的对照相比，转染miR-125 b模拟物后的HGC-27细胞的增殖显著增加，凋亡显著减少（P <0.05）。因此，miR-125 b可能作为癌基因在胃癌中通过失调胃细胞增殖和凋亡发挥作用。
MicroRNAs exert regulatory effects on a number of genes, thereby contributing to both physiological and pathological processes. The functions of microRNAs in tumorigenesis are becoming increasingly clear. In the present study, we investigated the role of microRNA-125b (miR‑125b), previously implicated in prostate and breast cancer, in gastric cancer, particularly regarding proliferation and apoptosis of gastric cancer cells. The expression of miR‑125b was measured in 50 samples of gastric cancer tissues and corresponding para-cancerous tissues by real-time PCR. The levels of miR‑125b expression in the gastric cancer tissues were significantly higher compared to the adjacent normal tissues (P<0.05). To begin to understand how the increased expression of miR‑125b may promote gastric cancer, the miR‑125b mimic was transfected into the gastric cancer cell line, HGC‑27, for the determination of proliferation (CCK8) and apoptosis (Annexin V) by flow cytometry. The results demonstrated that the proliferation significantly increased and apoptosis significantly decreased in the HGC‑27 cells following transfection with the miR‑125b mimic, compared to the untreated and scramble‑treated controls (P<0.05). Thus, miR‑125b may act as an oncogene in gastric cancer by dysregulating gastric cell proliferation and apoptosis.