Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects.
Heat-Shock Protein Expression in Mytilus californianus: Acclimatization (Seasonal and Tidal-Height Comparisons) and Acclimation Effects.
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DOI：
10.2307/1542724
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发表时间：
1997-04
期刊：
影响因子：
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通讯作者：
D. Roberts;Gretchen E Hofmann;A. G. N. Somero
中科院分区：
文献类型：
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作者：
D. Roberts;Gretchen E Hofmann;A. G. N. Somero
研究了俄勒冈州沿海的加州贻贝（Mytilus californianus）在野外驯化和实验室驯化后鳃中热休克蛋白（hsp）的表达。内源性水平的热休克蛋白在70 kDa的类（热休克蛋白70亚型）和配置文件的诱导温度为新合成的热休克蛋白70测量新鲜现场采集的标本作为功能的位置高度在潮间带和季节，并在贻贝7周后的实验室热驯化。内源hsp70水平随季节和采集高度的变化存在显著差异。新的热休克蛋白70的合成强烈诱导发生在现场标本测量范围内的体温。热休克蛋白70热诱导的配置文件随季节变化显着，但不与高度收集。在冬季和夏季驯化的贻贝之间的热休克蛋白70表达的巨大差异相反，在不同的驯化贻贝没有发生与温度相关的差异。野外习服和实验室热习服的差异表明，应激反应除了受体温的影响外，还受环境因素的影响。因此，在从实验室驯化研究推断田间种群的驯化效应时需要谨慎。在热休克反应的季节性和潮汐高度变化的背景下，蛋白质周转的能量成本进行了讨论。
Heat-shock protein (hsp) expression was examined in gill of field-acclimatized and laboratory-acclimated mussels (Mytilus californianus) from the Oregon coast. Endogenous levels of heat-shock proteins in the 70-kDa class (hsp70 isoforms) and profiles of induction temperature for newly synthesized hsp 70 were measured in freshly field-collected specimens as functions of location height in the intertidal and season, and in mussels after 7 weeks of laboratory thermal acclimation. There were significant differences in endogenous levels of hsp70 as functions of season and collection height. Strong induction of new hsp70 synthesis occurred at body temperatures within the range measured in field specimens. Profiles of hsp70 thermal induction varied significantly with season, but not with height of collection. In contrast to the large differences in hsp70 expression between winter- and summer-acclimatized mussels, no differences related to temperature occurred in the differently acclimated mussels. The differences found between the effects of field acclimatization and laboratory thermal acclimation suggest that the stress response is modulated by environmental factors in addition to body temperature. Thus, caution is required in extrapolating from laboratory acclimation studies to acclimatization effects in field populations. The seasonal and tidal-height variations in the heat-shock response are discussed in the context of energy costs of protein turnover.