Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture
Creating Cartilage in Tissue-Engineered Chamber Using Platelet-Rich Plasma Without Cell Culture
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使用富含血小板的血浆在组织工程室中创建软骨，无需细胞培养
DOI：
10.1089/ten.tec.2020.0049
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Lei Chen
中科院分区：
文献类型：
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作者：
Wang Meishui;Chen Guojie;Li Guanmin;Wang Biao;Lei Chen
背景：临床迫切需要大体积组织工程软骨等临床可用软骨。组织工程室（TEC）模型是一种很有前途的器官水平策略，可以在腔室内有效地扩大细胞或组织。传统的TEC技术不适合软骨培养，因为它缺乏必要的软骨生长因子，这种因子存在于富血小板血浆（PRP）中。在这项研究中，我们在TEC中加入了与PRP混合的自体耳廓软骨碎片，以获得大量的工程软骨。实验：为验证该方法的有效性，将48只新西兰大白兔随机分为4组：PRP组、血管化组（Ves）、PRP组、PRP+Ves组和对照组。取兔耳软骨，切成2 mm的碎片，然后注射到tec中。软骨构建体于第8周收获，并测量构建体体积。组织形态、免疫化学染色和机械强度进行评估。结果：第8周，PRP+Ves构建体呈现白色软骨样外观。软骨体积从原来的0.30 mL增加到1.8±0.1789 mL，增加了600%。组织学染色显示PRP组和PRP+Ves组包埋软骨边缘软骨细胞增生。此外，PRP+Ves组的软骨构建物表现出与正常软骨相似的力学特征。结论：兔耳软骨碎片掺入PRP和TEC血管化后，8周后软骨组织体积显著增加。耳部软骨组织缺损的修复一直是整形外科医生面临的巨大挑战。本文提出了一种在组织工程室中不进行细胞培养的情况下，在8周内产生耳廓软骨的新方法。拥有这样的方法是朝着创造大量功能性软骨组织迈出的有价值的一步，这可能导致以最小的供体部位发病率成功构建正常的耳廓结构。
Background:Clinically available cartilage, such as large-volume tissue-engineered cartilage, is urgently required for various clinical applications. Tissue engineering chamber (TEC) models are a promising organ-level strategy for efficient enlargement of cells or tissues within the chamber. The conventional TEC technology is not suitable for cartilage culture, because it lacks the necessary chondrogenic growth factor, which is present in platelet-rich plasma (PRP). In this study, we added autogenous auricular cartilage fragments mixed with PRP in a TEC to obtain a large amount of engineered cartilage.Experiment:To prove the efficacy of this method, 48 New Zealand white rabbits were randomly divided into 4 groups: PRP, vascularized (Ves), PRP, PRP+Ves, and control. Auricular cartilage was harvested from the rabbits, cut into fragments (2 mm), and then injected into TECs. Cartilage constructs were harvested at week 8, and construct volumes were measured. Histological morphology, immunochemical staining, and mechanical strength were evaluated.Results:At week 8, PRP+Ves constructs developed a white, cartilage-like appearance. The volume of cartilage increased by 600% the original volume from 0.30 to 1.8 ± 0.1789 mL. Histological staining showed proliferation of edge chondrocytes in the embedded cartilage in the PRP and PRP+Ves groups. Furthermore, the cartilage constructs in the PRP+Ves group show mechanical characteristics similar to those of normal cartilage.Conclusions:Auricular cartilage fragments mixed with PRP and vascularization of the TEC showed a significantly increased cartilage tissue volume after 8 weeks of incubation in rabbits.Impact StatementRepair of defects of ear cartilage tissue has always been a huge challenge to plastic surgeons. In this article, a new method is presented to produce within 8 weeks auricular cartilage in a tissue engineering chamber without cell culture. Having such a method is a valuable step toward creating a large volume of functional cartilage tissue, which may lead to successful construction of normal auricular structure with minimal donor-site morbidity.