The chloroplast genome from a lycophyte (microphyllophyte), Selaginella uncinata, has a unique inversion, transpositions and many gene losses
The chloroplast genome from a lycophyte (microphyllophyte), Selaginella uncinata, has a unique inversion, transpositions and many gene losses
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DOI：
10.1007/s10265-006-0055-y
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发表时间：
2007-03-01
影响因子：
2.8
通讯作者：
Yamaguchi, Kazuo
中科院分区：
文献类型：
--
作者：
Tsuji, Sumika;Ueda, Kunihiko;Yamaguchi, Kazuo
我们测定了卷柏叶绿体基因组的全序列，该植物属于维管植物的基本谱系。环状双链DNA全长144,170 bp，反向重复序列长25,578bp，中间隔着77,706 bp的大单复制区(LSC)和40,886bp的小单复制区(SSC)。我们指定了81个蛋白质编码基因，其中包括4个假基因，4个rRNA基因，只有12个tRNA基因。在大多数陆地植物叶绿体基因组中发现的四个基因rps 15、rps 16、rpl32和ycf10在钩吻海棠中不存在。另一种石杉的叶绿体基因组的基因序列和排列与苔藓植物的几乎相同，而钩毛石杉的叶绿体基因组的基因序列和排列与苔藓植物的典型结构有很大的不同，这是因为在LSC中存在一个独特的20kb的倒位，两个片段从LSC到SSC的转位和许多基因丢失。因此，uncinata叶绿体基因组的组织为了解大约4亿年前从真叶植物中分离出来的番茄的进化提供了新的视角。
We determined the complete nucleotide sequence of the chloroplast genome of Selaginella uncinata, a lycophyte belonging to the basal lineage of the vascular plants. The circular double-stranded DNA is 144,170 bp, with an inverted repeat of 25,578 bp separated by a large single copy region (LSC) of 77,706 bp and a small single copy region (SSC) of 40,886 bp. We assigned 81 protein-coding genes including four pseudogenes, four rRNA genes and only 12 tRNA genes. Four genes, rps15, rps16, rpl32 and ycf10, found in most chloroplast genomes in land plants were not present in S. uncinata. While gene order and arrangement of the chloroplast genome of another lycophyte, Hupertzia lucidula, are almost the same as those of bryophytes, those of S. uncinata differ considerably from the typical structure of bryophytes with respect to the presence of a unique 20 kb inversion within the LSC, transposition of two segments from the LSC to the SSC and many gene losses. Thus, the organization of the S. uncinata chloroplast genome provides a new insight into the evolution of lycophytes, which were separated from euphyllophytes approximately 400 million years ago.