Karyotyping and identifying all the chromosomesof allopolyploid Brassica juncea using multi-color FISH
Karyotyping and identifying all the chromosomesof allopolyploid Brassica juncea using multi-color FISH
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异源多倍体芥菜所有染色体的核型分析及多色FISH鉴定
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Zhiyong Xiong
中科院分区：
文献类型：
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作者：
Zhijun Xu;Bei Xie;Tian Wu;Xiaoxia Xin;Lingyu Mang;Guangxuan Tan;Zhiyong Xiong
利用荧光原位杂交(FISH)进行染色体鉴定和核型分析，为基因组和细胞遗传学研究提供了技术平台。芥菜(Brassica ciea，棕色芥菜，2n=4×=36；基因组AABB)是甘蓝型油菜(Brassica Rapa)与黑芥型油菜(Brassica Ngra)自发杂交的异源多倍体物种，含有许多有价值的品系。对芥菜染色体上的Thedistributionpatternsandchromosomallocalizationsofsixrepeatsequences.(satelliterepeatpBrSTR，5SrDNA，45SrDNA，B基因组特异重复序列BNBH35和着丝粒卫星重复序列CentBr1和CentBr2进行了分析，比较核型分析表明，与其二倍体祖先种相比，该基因组相对稳定，并揭示了芥菜种内核型的多样性。本研究为芥菜基因组进化提供了有价值的信息，并为染色体鉴定提供了有用的工具。
Chromosome identification and karyotype using fluorescence in situ hybridization (FISH).provides a technical platform for genome and cytogenetic studies. Brassica juncea (brown.mustard, 2n = 4× = 36; genome AABB) is an allopolyploid species that originated from a.spontaneous hybridization of Brassica rapa and Brassica nigra and contains many valuable.traits.Inthisstudy,amulticolorFISHprocedureallowingtheidentificationofall18chromosomal.pairs was developed by two-step hybridizations with probes on the same metaphase.chromosomes. Thedistributionpatternsandchromosomallocalizationsofsixrepeatsequences.(satelliterepeatpBrSTR,5SrDNA,45SrDNA,Bgenome-specificrepeatpBNBH35,andcentromeric.satellite repeats CentBr1 and CentBr2) on B. juncea chromosomes were characterized..Comparative karyotype analyses showed that the genome is relatively stable in comparison.with its diploid progenitor species and revealed intraspecific karyotypic diversity among three.accessions of B. juncea. This study provides valuable information about the genome evolution of.B. juncea and a toolkit that will be helpful for chromosome identification