Sequence variation in the two-component histidine kinase gene of Botrytis cinerea associated with resistance to dicarboximide fungicides
Sequence variation in the two-component histidine kinase gene of Botrytis cinerea associated with resistance to dicarboximide fungicides
复制标题
灰葡萄孢双组分组氨酸激酶基因序列变异与二甲酰亚胺类杀菌剂抗性相关
DOI：
10.1016/j.pestbp.2007.01.005
复制
发表时间：
2007-07-01
影响因子：
4.7
通讯作者：
Michallides, Themis J.
中科院分区：
文献类型：
--
作者：
Ma, Zhonghua;Yan, Leiyan;Michallides, Themis J.
The complete two-component histidine kinase gene (Bosl) was sequenced from eight dicarboximide-resistant (D-R) and six-sensitive (D-S) field isolates of Botrytis cinerea. Comparisons in the predicted amino acid sequences of Bosl showed that each two D-R isolates had a single point mutation at amino acid position 365 from an isoleucine to serine (I365S) or to an asparagine (I365N). Three D-R isolates were characterized with a change from glutamine to proline at position 369 (Q369P) as well as an asparagine to serine at the position 373 (N373S). These mutations located within the 90-amino-acid repeats of Bos1 have been reported previously. One new mutation, however, was found in the D-R isolate 65-E8. In this isolate, a null mutation at the amino acid position 1040 in the Bosl was detected. Inoculation tests showed that this isolate was nearly nonpathogenic to cucumber. After the Bosl gene from the sensitive isolate 38B1 was transferred into the resistant isolate 65-E8, all transformants tested were sensitive to iprodione and capable of infecting cucumber. DNA fingerprint generated by micro-satellite primed-PCR showed that isolates, were not clustered based on their sensitivity to iprodione. Results from this study indicate that mutations in the Bos1 gene associated with dicarboximide resistance are diverse in B. cinerea, and the Bosl gene is associated with virulence of B. cinerea. (c) 2007 Elsevier Inc. All rights reserved.
