Bitter Melon Enhances Natural Killer-Mediated Toxicity against Head and Neck Cancer Cells.
Bitter Melon Enhances Natural Killer-Mediated Toxicity against Head and Neck Cancer Cells.
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DOI：
10.1158/1940-6207.capr-17-0046
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发表时间：
2017-06
期刊：
影响因子：
--
通讯作者：
Ray RB
中科院分区：
文献类型：
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作者：
Bhattacharya S;Muhammad N;Steele R;Kornbluth J;Ray RB
自然杀伤（NK）细胞是先天免疫的主要组成部分之一，具有介导抗肿瘤活性的能力。对NK细胞介导的肿瘤杀伤在控制实体瘤生长中的作用的认识仍处于发展阶段。我们最近发现，苦瓜提取物（BME）调节性T细胞（Treg）在头颈部鳞状细胞癌（HNSCC）的人口。然而，BME在针对HNSCC的NK细胞调节中的作用仍然未知。在这项研究中，我们研究了BME是否可以增强NK细胞对HNSCC细胞的杀伤活性。我们的结果表明，用BME处理人NK细胞系（NK3.3）增强了杀死HNSCC细胞的能力。在暴露于BME的NK 3.3细胞中，BME增加颗粒酶B积累和CD 107 a/LAMP 1的易位/积累。此外，当与HNSCC细胞共培养时，观察到BME处理的NK3.3细胞中CD 16和NKp 30的细胞表面表达增加。总的来说，我们的结果首次证明BME增强NK细胞介导的HNSCC杀伤活性，暗示BME的免疫调节作用。
Natural Killer (NK) cells are one of the major components of innate immunity, with the ability to mediate antitumor activity. Understanding the role of NK cell mediated tumor killing in controling of solid tumor growth is still in the developmental stage. We have shown recently that bitter melon extract (BME) modulates the regulatory T cell (Treg) population in head and neck squamous cell carcinoma (HNSCC). However, the role of BME in NK cell modulation against HNSCC remains unknown. In this study, we investigated whether BME can enhance the NK cell killing activity against HNSCC cells. Our results indicated that treatment of human NK cell line (NK3.3) with BME enhances ability to kill HNSCC cells. BME increases granzyme B accumulation and translocation/accumulation of CD107a/LAMP1 in NK3.3 cells exposed to BME. Further, an increase in cell surface expression of CD16 and NKp30 in BME treated NK3.3 cells was observed when co-cultured with HNSCC cells. Collectively, our results demonstrated for the first time that BME augments NK cell mediated HNSCC killing activity, implicating an immunomodulatory role of BME.