CBL1, a calcium sensor that differentially regulates salt, drought, and cold responses in Arabidopsis
CBL1, a calcium sensor that differentially regulates salt, drought, and cold responses in Arabidopsis
复制标题
DOI：
10.1105/tpc.012393
复制
发表时间：
2003-08-01
期刊：
影响因子：
11.6
通讯作者：
Luan, S
中科院分区：
文献类型：
--
作者：
Cheong, YH;Kim, KN;Luan, S
虽然钙离子是高等植物逆境胁迫基因表达的信号转导途径中的重要组成部分，但对钙离子功能的分子机制知之甚少。据信，细胞钙的变化被传感器分子感知，包括钙结合蛋白。钙调磷酸酶B样（CBL）蛋白家族代表了植物中一组独特的钙传感器。该家族的一个成员CBL1可被多种应激信号高度诱导，暗示CBL1参与应激反应途径。当转基因拟南芥中CBL1蛋白水平增加时，它改变了这些植物的胁迫反应途径。虽然干旱诱导的基因表达增强，基因诱导冷抑制。此外，CBL1过表达的植物表现出对盐和干旱的耐受性增强，但对冷冻的耐受性降低。与此相反，cbl1无效突变体植物表现出增强的低温诱导和减少干旱诱导的胁迫基因。突变体表现出较低的耐盐性和耐旱性，但增强了耐冷冻性。这些研究表明，CBL1在植物中作为盐和干旱响应的正调节器和冷响应的负调节器发挥作用。
Although calcium is a critical component in the signal transduction pathways that lead to stress gene expression in higher plants, little is known about the molecular mechanism underlying calcium function. It is believed that cellular calcium changes are perceived by sensor molecules, including calcium binding proteins. The calcineurin B-like (CBL) protein family represents a unique group of calcium sensors in plants. A member of the family, CBL1, is highly inducible by multiple stress signals, implicating CBL1 in stress response pathways. When the CBL1 protein level was increased in transgenic Arabidopsis plants, it altered the stress response pathways in these plants. Although drought-induced gene expression was enhanced, gene induction by cold was inhibited. In addition, CBL1-overexpressing plants showed enhanced tolerance to salt and drought but reduced tolerance to freezing. By contrast, cbl1 null mutant plants showed enhanced cold induction and reduced drought induction of stress genes. The mutant plants displayed less tolerance to salt and drought but enhanced tolerance to freezing. These studies suggest that CBL1 functions as a positive regulator of salt and drought responses and a negative regulator of cold response in plants.