Voluntary running provides neuroprotection in rats after 6-hydroxydopamine injection into the medial forebrain bundle
Voluntary running provides neuroprotection in rats after 6-hydroxydopamine injection into the medial forebrain bundle
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DOI：
10.1023/b:mebr.0000027416.13070.c3
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发表时间：
2004-06-01
影响因子：
3.6
通讯作者：
Russell, VA
中科院分区：
文献类型：
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作者：
Mabandla, M;Kellaway, L;Russell, VA
神经毒性药物，如6-羟基多巴胺(6-OHDA)，已被用于在大鼠模型中模拟帕金森状态。在单侧注射6-OHDA后立即被迫使用受损肢体的大鼠中，6-OHDA的毒性效应已被证明是减轻的。这项研究的目的是确定自愿锻炼的大鼠黑质中的多巴胺神经元是否幸免于难。两组大鼠均于前脑内侧束注射6-OHDA(10-MUG/4 MU生理盐水)前7天，放入装有转轮的笼子内。在研究期间，对照组的跑轮被固定。在注射6-OHDA后，将大鼠放回各自的笼子中，并在笼子里继续停留14天。试验组每天记录自由跑时的车轮转数。实验结束后，皮下注射阿朴吗啡(0.5 mg/kg，S.C.)。并记录旋转次数和旋转方向。在跑轮上运动的大鼠对阿朴吗啡注射的反应没有反向旋转，这表明多巴胺神经元已经充分避免了6-OHDA注射的毒性影响，以防止纹状体突触后多巴胺受体的上调。
Neurotoxic drugs such as 6-hydroxydopamine (6-OHDA) have been used to mimic a Parkinsonian state in a rat model. The toxic effect of 6-OHDA has been shown to be reduced in rats that were forced to use the impaired limb immediately after unilateral 6-OHDA injection. The aim of this study was to determine whether dopamine neurons in the substantia nigra are spared in rats that exercise voluntary. Two groups of rats were placed in cages with attached running wheels 7 days prior to injection of 6-OHDA (10 mug/4 muL saline) into the medial forebrain bundle. The running wheels of the control group were immobilized for the duration of the study. After 6-OHDA injection, the rats were returned to their respective cages where they remained for a further period of 14 days. Wheel revolutions during free running were recorded daily in the experimental group. At the end of this period the rats were injected with apomorphine (0.5 mg/kg, s.c.) and the number and direction of rotations was recorded. Rats that exercised in the running wheels did not rotate contralaterally in response to apomorphine injection, suggesting that dopamine neurons had been spared sufficiently from the toxic effects of 6-OHDA injection to prevent upregulation of postsynaptic dopamine receptors in the striatum.