ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana
ESTs reveal a multigene family for plant defensins in Arabidopsis thaliana
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DOI：
10.1016/s0014-5793(96)01378-6
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发表时间：
1997-01-03
期刊：
影响因子：
3.5
通讯作者：
Bohlmann, H
中科院分区：
文献类型：
--
作者：
Epple, P;Apel, K;Bohlmann, H
植物防御素，以前被称为γ -硫蛋白，是一组小的，富含半胱氨酸的碱性和抗菌植物蛋白。对Arabinopsis thaliana表达序列标签（est）的随机测序显示，该植物物种中有几个不同的植物防御素基因，可分为两个亚科。我们利用每个亚家族的一个EST来研究拟南芥中相应基因的表达。Pdf2.3在幼苗、莲座、花和硅橡胶中组成性表达，并且不能被茉莉酸甲酯、水杨酸酯、乙烯利和硝酸银或几种不同的植物致病真菌诱导。第二种基因Pdf1.2的表达在未经处理的植物中只在莲座中检测到。在幼苗中，它可以被茉莉酸甲酯、硝酸银和不同的植物致病真菌诱导，尤其是镰刀菌（Fusarium oxysporum f.s. matthiolae）。Pdf1.2的调控类似于病原体诱导的硫蛋白基因Thi2.1。
Plant defensins, formerly named gamma-thionins, are a group of small, cysteine-rich, basic, and antimicrobial plant proteins, Random sequencing of expressed sequence tags (ESTs) in Arabinopsis thaliana has revealed several different plant defensin genes in this plant species which can be grouped into two subfamilies. We have used one EST of each subfamily to study the expression of the corresponding genes in A. thaliana. Pdf2.3 is constitutively expressed in seedlings, rosettes, flowers, and siliques and is not inducible in seedlings either by methyl jasmonate, salicylate, ethephon, and silver nitrate or by several different phytopathogenic fungi. The expression of a second gene, Pdf1.2, is in untreated plants only detectable in rosettes. In seedlings, it is inducible by methyl jasmonate, silver nitrate, and different phytopathogenic fungi, notably Fusarium oxysporum f. sp. matthiolae. The regulation of Pdf1.2 resembles that of the pathogen-inducible thionin gene Thi2.1.