Characterizing blood microparticles: technical aspects and challenges.
Characterizing blood microparticles: technical aspects and challenges.
复制标题
DOI：
10.2147/vhrm.s955
复制
发表时间：
2008
影响因子：
2.9
通讯作者：
Shet AS
中科院分区：
文献类型：
--
作者：
Shet AS
Although long considered to be cellular debris, microparticles (MPs) are more recently considered reflective of cellular stimulation, activation, and degeneration/apoptosis. MPs that arise from the cellular components of blood and the endothelial lining of blood vessels are referred to as blood MPs and by general consensus are small (≤1.5 μm), expose the anionic phospholipid (PL) phosphatidylserine (PS) on the outer leaflet of their membrane, and bear surface membrane antigens reflecting their cellular origin. This brief review summarizes the different approaches used by several groups to study blood MPs. The aim of this article is to review the technical aspects of characterizing the morphological and functional properties of blood MPs with emphasis on the preanalytical and analytical variables involved in these studies.
