Genome-Wide Identification and Characterization of Actin-Depolymerizing Factor (ADF) Family Genes and Expression Analysis of Responses to Various Stresses in Zea Mays L.
Genome-Wide Identification and Characterization of Actin-Depolymerizing Factor (ADF) Family Genes and Expression Analysis of Responses to Various Stresses in Zea Mays L.
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DOI：
10.3390/ijms21051751
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Zhai, Lihong
中科院分区：
文献类型：
--
作者：
Huang, Jun;Sun, Wei;Zhai, Lihong
肌动蛋白解聚因子（ADF）是一小类肌动蛋白结合蛋白，调节细胞内肌动蛋白的动力学。此外，众所周知，植物ADF家族在生长、发育和防御相关功能中起着关键作用。结果：13个玉米（Zea mays L.，使用隐马尔可夫模型鉴定ADF基因。系统发育分析表明，小立碗藓、拟南芥、水稻和玉米中已鉴定的36个ADF基因可聚为5类。在玉米ADF基因家族中发现了4对节段基因。使用从玉米和水稻eFP浏览器获得的微阵列数据分析ZmADF和OsADF的组织特异性表达。来自第V组的5个ZmADF（ZmADF 1/2/7/12/13）在雄穗、花粉和花药中特异性高表达。利用qRT-PCR技术分析了13个ZmADFs在5种非生物胁迫下的表达模式，发现ADFs主要响应热、盐、干旱和阿坝胁迫。结论：本研究鉴定了玉米ADF基因，并分析了基因结构和系统发育关系。表达分析结果表明，ADF基因在不同组织和不同胁迫（包括非生物胁迫和激素胁迫）中的表达水平不同，表明其在植物生长发育和对外界刺激的反应中具有不同的作用。本报告扩展了我们的知识，以了解玉米ADF基因的功能。
Actin-depolymerizing factor (ADF) is a small class of actin-binding proteins that regulates the dynamics of actin in cells. Moreover, it is well known that the plant ADF family plays key roles in growth, development and defense-related functions. Results: Thirteen maize (Zea mays L., ZmADFs) ADF genes were identified using Hidden Markov Model. Phylogenetic analysis indicated that the 36 identified ADF genes in Physcomitrella patens, Arabidopsis thaliana, Oryza sativa japonica, and Zea mays were clustered into five groups. Four pairs of segmental genes were found in the maize ADF gene family. The tissue-specific expression of ZmADFs and OsADFs was analyzed using microarray data obtained from the Maize and Rice eFP Browsers. Five ZmADFs (ZmADF1/2/7/12/13) from group V exhibited specifically high expression in tassel, pollen, and anther. The expression patterns of 13 ZmADFs in seedlings under five abiotic stresses were analyzed using qRT-PCR, and we found that the ADFs mainly responded to heat, salt, drought, and ABA. Conclusions: In our study, we identified ADF genes in maize and analyzed the gene structure and phylogenetic relationships. The results of expression analysis demonstrated that the expression level of ADF genes was diverse in various tissues and different stimuli, including abiotic and phytohormone stresses, indicating their different roles in plant growth, development, and response to external stimulus. This report extends our knowledge to understand the function of ADF genes in maize.