A Lab Assembled Microcontroller-Based Sensor Module for Continuous Oxygen Measurement in Portable Hypoxia Chambers.
A Lab Assembled Microcontroller-Based Sensor Module for Continuous Oxygen Measurement in Portable Hypoxia Chambers.
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DOI：
10.1371/journal.pone.0148923
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Szerlip NJ
中科院分区：
文献类型：
--
作者：
Mathupala SP;Kiousis S;Szerlip NJ
基于缺氧的细胞培养实验是体外癌症研究的常规和必要组成部分。大多数实验室使用低成本的便携式模块化腔室来实现细胞培养的缺氧条件，其中密封腔室用预设O2浓度的气体混合物吹扫。研究是在这样的假设下进行的，即缺氧在整个48至72小时的实验持续时间内保持不变。由于这些室缺乏任何传感器或检测系统来监测气相O2，基于细胞的数据往往是不均匀的，这是由于实验设置的特殊性质。随着低成本开源的基于微处理器的电子项目套件的可用性，研究人员现在可以使用易于使用的软件对其进行编程，将其连接到传感器，并将其放置在基本的科学仪器中以监测和记录实验参数。我们在这里报告的设计和建造的一个小的足迹工具包连续测量和记录的氧气浓度在模块化缺氧室。低成本组件（135美元）由基于Arduino的微控制器，数据记录免费软件和工厂预校准的微型O2传感器组成。作者编写了一个小而直观的软件程序来控制数据的输入和输出。该工具包的基本性质将使任何学生在生物学与业余电子经验最少的组装系统和编辑程序参数，以适应个人的实验条件。我们通过一系列的缺氧实验来展示该试剂盒的实用性和数据输出的稳定性。研究还表明，迫切需要在缺氧实验过程中监测和调整气相O2浓度，以防止由于部分缺氧而导致的实验错误或失败。因此，将传感器-微控制器模块结合到便携式低氧室为研究人员提供了以前仅可用于使用复杂（且昂贵）细胞培养孵育器的实验室的能力。
Hypoxia-based cell culture experiments are routine and essential components of in vitro cancer research. Most laboratories use low-cost portable modular chambers to achieve hypoxic conditions for cell cultures, where the sealed chambers are purged with a gas mixture of preset O2 concentration. Studies are conducted under the assumption that hypoxia remains unaltered throughout the 48 to 72 hour duration of such experiments. Since these chambers lack any sensor or detection system to monitor gas-phase O2, the cell-based data tend to be non-uniform due to the ad hoc nature of the experimental setup. With the availability of low-cost open-source microcontroller-based electronic project kits, it is now possible for researchers to program these with easy-to-use software, link them to sensors, and place them in basic scientific apparatus to monitor and record experimental parameters. We report here the design and construction of a small-footprint kit for continuous measurement and recording of O2 concentration in modular hypoxia chambers. The low-cost assembly (US$135) consists of an Arduino-based microcontroller, data-logging freeware, and a factory pre-calibrated miniature O2 sensor. A small, intuitive software program was written by the authors to control the data input and output. The basic nature of the kit will enable any student in biology with minimal experience in hobby-electronics to assemble the system and edit the program parameters to suit individual experimental conditions. We show the kit’s utility and stability of data output via a series of hypoxia experiments. The studies also demonstrated the critical need to monitor and adjust gas-phase O2 concentration during hypoxia-based experiments to prevent experimental errors or failure due to partial loss of hypoxia. Thus, incorporating the sensor-microcontroller module to a portable hypoxia chamber provides a researcher a capability that was previously available only to labs with access to sophisticated (and expensive) cell culture incubators.