A Novel Method for Frictional Testing on Adherent Cells
A Novel Method for Frictional Testing on Adherent Cells
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贴壁细胞摩擦测试的新方法
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
R. Tran‐Son‐Tay
中科院分区：
文献类型：
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作者：
J. Cobb;A. C. Dunn;J. Kwan;M. Sarntinoranont;G. Sawyer;R. Tran‐Son‐Tay
了解生物材料和体内组织之间的物理相互作用对于医疗设备的开发至关重要。例如，两者之间的摩擦系数必须足够低，以避免损坏构成组织的细胞。因此，需要一种获取细胞摩擦数据的方法。在目前的工作中，人角膜上皮（HCE-T）细胞在专门的细胞支架中培养，并使用微型摩擦计进行摩擦实验。通过显微镜和台盼蓝活力测定评估细胞损伤。初步实验提供了完整的 HCE-T 单层被微摩擦磨损试验机的玻璃针磨损的数据。玻璃针在完整细胞层上摩擦的摩擦系数为 0.037 ± 0.016。此外，这些数据显示获得的摩擦系数与发生的细胞损伤量之间存在直接相关性。迄今为止开发的方案可用于对各种贴壁细胞类型（例如上皮细胞、内皮细胞等）进行摩擦测试。此外，可以修改微摩擦计的设置，以获得细胞和各种生物材料之间的摩擦系数测量。版权所有 © 2007 ASME
Understanding the physical interaction between biomaterials and tissues within the body is critical for the development of medical devices. For instance, the coefficient of friction between the two must be low enough to avoid damaging the cells that make up the tissue. Therefore, a method for obtaining friction data on cells is needed. In the present work, human corneal epithelial (HCE-T) cells are cultured within specialized cell holders and friction experiments are performed with a micro-tribometer. Cell damage is assessed via microscopy and a trypan blue viability assay. Preliminary experiments provide data for an intact monolayer of HCE-Ts being abraded by the glass pin of the micro-tribometer. The coefficient of friction for the glass pin rubbing on an intact cell layer is 0.037 ± 0.016. Additionally, these data show a direct correlation between the friction coefficients obtained and the amount of cell damage that occurs. The protocols developed thus far can be used to conduct friction testing on various adherent cell types (e.g.epithelial cells, endothelial cells, etc.). Furthermore, the micro-tribometer set-up can be modified in order to obtain friction coefficient measurements between cells and various biomaterials.Copyright © 2007 by ASME