STAT5A Modulates Chemokine Receptor CCR6 Expression and Enhances Pre‐B Cell Growth in a CCL20‐Dependent Manner
STAT5A Modulates Chemokine Receptor CCR6 Expression and Enhances Pre‐B Cell Growth in a CCL20‐Dependent Manner
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DOI：
10.1002/jcb.25558
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发表时间：
2016-11
影响因子：
4
通讯作者：
T. Tsuruyama;T. Hiratsuka;Wulamujiang Aini;Takuro Nakamura
中科院分区：
文献类型：
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作者：
T. Tsuruyama;T. Hiratsuka;Wulamujiang Aini;Takuro Nakamura
在先天免疫中，信号换能器和转录激活因子5A （STAT5A）通过抑制细胞因子信号（Socs）基因参与B细胞对细胞因子的应答。然而，其在B细胞对趋化因子反应中的直接作用尚不清楚。在这项研究中，我们研究了STAT5A在先天免疫应答中的作用。我们发现STAT5A上调C‐C基序受体6 （Ccr6）的转录，诱导对其配体CCL20的应答。STAT5A转录活性通过结合前B细胞基因组中CCR6启动子中的干扰素γ激活位点（GAS）元件进行。高水平的STAT5A和CCR6增加了前B细胞依赖CCL20的集落生长。在伴有炎症的人B淋巴母细胞淋巴瘤中，STAT5A磷酸化与CCR6表达相关（与无炎症的患者相比，P < 0.05）。总之，我们的数据支持了我们的假设，即STAT5A增强了前B细胞对CCL20的反应，从而促进了它们的生长。j .细胞。中国生物医学工程学报，2016,31(2):444 - 444。©2016 Wiley期刊公司
Signal transducer and activator of transcription 5A (STAT5A) contributes to B‐cell responses to cytokines through suppressor of cytokine signaling (Socs) genes in innate immunity. However, its direct roles in B‐cell responses to chemokines are poorly understood. In this study, we examined the role of STAT5A in the innate immune response. We found that STAT5A upregulated the transcription of C‐C motif receptor 6 (Ccr6) to induce responses to its ligand, CCL20. STAT5A transcriptional activity proceeded through binding to the interferon‐γ activation site (GAS) element in the CCR6 promoter in the genome of pre‐B cells. High levels of STAT5A and CCR6 increased CCL20‐dependent colony growth of pre‐B cells. In human B‐lymphoblastic lymphoma with inflammation, STAT5A phosphorylation was correlated with CCR6 expression (P > 0.05 compared with that in cases without inflammation). In conclusion, our data supported our hypothesis that STAT5A enhanced the response of pre‐B cells to CCL20 to promote their growth. J. Cell. Biochem. 117: 2630–2642, 2016. © 2016 Wiley Periodicals, Inc.