Characterization of a Highly Virulent Noncytopathic Bovine Viral Diarrhea Virus 2b Isolate Detected in Cattle in Inner Mongolia, China
Characterization of a Highly Virulent Noncytopathic Bovine Viral Diarrhea Virus 2b Isolate Detected in Cattle in Inner Mongolia, China
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DOI：
10.1155/2023/3821435
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发表时间：
2023-03-10
影响因子：
4.3
通讯作者：
Wang，Wei
中科院分区：
文献类型：
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作者：
Chai，Chunxia;Zhang，Fan;Wang，Wei
牛病毒性腹泻病毒（BVDV）感染在世界范围内引起牛的亚临床至严重急性疾病。识别了BVDV的两种基因型，BVDV 1和BVDV 2。目前，BVDV 1b和BVDV 2a亚型主要在中国流行。在此，从牛中分离并鉴定了一种新的非细胞病变（ncp）BVDV毒株，命名为BVDV VNM 21。测定了BVDV VNM 21全基因组序列，并对其5′非翻译区（5′UTR）、Npro和E2基因进行了系统发育分析，全基因组序列显示该病毒属于BVDV 2b亚型，在BVDV 2b内形成了一个独立的进化枝。全基因组序列分析表明，BVDV VNM 21与中国分离株SD 1301具有较高的同源性（98.10%）。在豚鼠和小牛的实验性感染研究中，它们都出现了相似的临床体征，包括持续3天以上的明显和长期发热反应以及白色血细胞（WBC）和血小板显著减少超过40%。豚鼠感染后表现出更明显的急性感染特征，感染后第1天WBC下降超过50%，不到3天即恢复正常。结果表明，BVDV VNM 21株对犊牛具有较高的毒力。这是首次证实在牛中分离出高致病性ncpBVDV 2b，以及建立BVDV-豚鼠模型。本研究为进一步研究和控制BVDV 2b在中国的流行提供了依据。
Bovine viral diarrhea virus (BVDV) infection causes subclinical to severe acute disease in cattle all over the world. Two genotypes of BVDV are recognized, BVDV1 and BVDV2. Currently, the subtypes of BVDV1b and BVDV2a are mainly circulating in China. Here, a newly noncytopathic (ncp) BVDV strain named BVDVNM21 was isolated and identified from cattle. We determined the complete genome sequence of BVDVNM21, phylogenetic analysis of 5′ untranslated region (5′UTR), Npro, and E2 gene, and complete genome showed the virus belongs to BVDV2b subtype and formed an independent clade within BVDV2b. Genome‐wide sequence analysis showed that BVDVNM21 had high homology with SD1301 (98.10%) isolated from China. In the experimental infection study of guinea pigs and calves, they all developed similar clinical signs, including a pronounced and prolonged febrile response lasting more than 3 days and pronounced reduction in white blood cells (WBC) and platelets of more than 40%. Moreover, guinea pigs showed more acute infection characteristics after infection, and WBC decreased by more than 50% at 1 day postinfection (dpi), and they recovered in less than 3 days. The results indicated that the BVDVNM21 strain showed high virulence in calves. It was the first confirmed isolation of a highly pathogenic ncpBVDV2b in cattle, as well as the establishment of the BVDV‐guinea pig model. This study may provide a basis for further research and control of the prevalence of BVDV2b in China.