Cross-linked surface-grafted glycopolymer for multivalent recognition of lectin.
Cross-linked surface-grafted glycopolymer for multivalent recognition of lectin.
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DOI：
10.1021/ac071453q
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发表时间：
2007-10
影响因子：
7.4
通讯作者：
Lei Yu;Mingchuan Huang;P. Wang;Xiangqun Zeng
中科院分区：
文献类型：
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作者：
Lei Yu;Mingchuan Huang;P. Wang;Xiangqun Zeng
合成了α-连接甘露糖缀合的丙烯酰胺单体。该单体通过自由基聚合与丙烯酰胺、交联剂和表面连接剂直接在金表面上聚合。具有活性碳-碳双键的表面连接基通过硫醇锚预固定在金表面上。因此，交联的甘露糖缀合聚合物薄层被接枝到金表面上。这种聚合物薄层对其目标凝集素伴刀豆球蛋白 A (Con A) 显示出高结合灵敏度和优异的选择性，超越了以前使用的线性糖聚合物和自组装乙二醇单层，并通过石英晶体微天平、原子力显微镜和表面等离振子共振技术进行了验证。在低至 5 x 10(-10) M 的浓度下观察到对 Con A 的显着响应。该响应与磷酸盐缓冲盐水中高达 10(-7) M 的 Con A 浓度成正比。交联聚合物的使用降低了碳水化合物结合位点之间的聚合物主链的柔性。因此，多价结合的构象熵的成本被最小化。测得如此制备的交联聚合物与Con A的结合常数在2.5×10(6)至3.2×10(6)M(-1)之间。这些值明显大于我们早期研究碳水化合物自组装单层时获得的值。除了碳水化合物-凝集素识别之外，还可以通过控制交联程度来实现额外的选择性。
An alpha-link mannose-conjugated acrylamide monomer was synthesized. This monomer was polymerized by free radical polymerization with acrylamide, a cross-linker, and a surface linker directly on the gold surface. The surface linker, with an active carbon-carbon double bond, was preimmobilized on the gold surface by the thiol anchor. Thus, a cross-linked mannose-conjugated polymer thin layer was grafted onto a gold surface. This thin layer of polymer showed high binding sensitivity and excellent selectivity to its target lectin, concanavalin A (Con A), surpassing the formerly used linear glycopolymer and self-assembled glycol monolayers, validated by the techniques of quartz crystal microbalance, atomic force microscopy, and surface plasmon resonance. Remarkable response was observed to Con A at a concentration as low as 5 x 10(-10) M. The response is proportional to the Con A concentration up to 10(-7) M in phosphate-buffered saline. The use of cross-linked polymer decreased the flexibility of the polymer backbone between the carbohydrate binding sites. Therefore, the cost of conformational entropy for multivalent binding was minimized. The binding constants of the so-prepared cross-linked polymer with Con A were measured to be between 2.5 x 10(6) and 3.2 x 10(6) M(-1). These values are significantly larger than that obtained in our early study with a carbohydrate self-assembled monolayer. In addition to the carbohydrate-lectin recognition, additional selectivity may be achieved by controlling the degree of cross-linking.