A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (Rin) locus
A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (Rin) locus
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DOI：
10.1126/science.1068181
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发表时间：
2002-04-12
期刊：
影响因子：
56.9
通讯作者：
Giovannoni, J
中科院分区：
文献类型：
--
作者：
Vrebalov, J;Ruezinsky, D;Giovannoni, J
含有成熟抑制剂（rin）突变的番茄植株产生的果实无法成熟。此外，林植物表现出萼片增大和花序确定性丧失。 rin 基因座的位置克隆揭示了两个串联的 MADS-box 基因（LeMADS-RIN 和 LeMADS-MC），其表达模式分别表明在果实成熟和萼片发育中的作用。 rin 突变改变了这两个基因的表达。基因抑制和突变互补表明 LeMADS-RIN 调节成熟，而 LeMADS-MC 影响萼片发育和花序决定性。 LeMADS-RIN 展示了植物 MADS-box 基因在农业上的重要功能，并提供了对成熟的非激素（发育）调节的分子洞察。
Tomato plants harboring the ripening-inhibitor (rin) mutation yield fruits that fail to ripen. Additionally, rin plants display enlarged sepals and loss of inflorescence determinacy. Positional cloning of the rin locus revealed two tandem MADS-box genes (LeMADS-RIN and LeMADS-MC), whose expression patterns suggested roles in fruit ripening and sepal development, respectively. The rin mutation alters expression of both genes. Gene repression and mutant complementation demonstrate that LeMADS-RIN regulates ripening, whereas LeMADS-MC affects sepal development and inflorescence determinacy. LeMADS-RIN demonstrates an agriculturally important function of plant MADS-box genes and provides molecular insight into nonhormonal (developmental) regulation of ripening.