Selective expression of a VHIV subfamily of immunoglobulin genes in human CD5+ B lymphocytes from cord blood.
Selective expression of a VHIV subfamily of immunoglobulin genes in human CD5+ B lymphocytes from cord blood.
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DOI：
10.1084/jem.174.1.109
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发表时间：
1991-07-01
影响因子：
15.3
通讯作者：
Lydyard, P M
中科院分区：
文献类型：
--
作者：
Mageed, R A;MacKenzie, L E;Stevenson, F K;Yuksel, B;Shokri, F;Maziak, B R;Jefferis, R;Lydyard, P M
表达 CD5 表面抗原的人 B 淋巴细胞（CD5+ B 细胞）构成了能够产生识别多种自身抗原的多特异性抗体的子集。 CD5+ 和 CD5- B 细胞产生的抗体库不同。然而，目前尚不清楚这种分布是否反映在不同的免疫球蛋白可变区基因（IgV）的使用中。重链 IgV (IgVH) 基因的重排是 B 细胞成熟过程中第一个可识别的阶段之一，并且以发育有序的方式发生。 IgVH 基因表达在胎儿时期受到高度限制，但在出生后逐渐多样化。先前已在人胎肝 B 细胞中证实了来自相对较小的 VHIV 基因家族的 VH 基因的高频率使用。在本研究中，使用交叉反应独特型 (CRI) 单克隆抗体检查了通过 Epstein-Barr 病毒转化分离的 CD5+ 和 CD5- 脐带血 B 细胞建立的 102 个 B 细胞系的 IgV 表达频率。结果表明，脐带血 B 细胞中 VHIV 基因的使用频率相对较高（30%）。此外，与 VHIV 家族不同亚群相关的两个相互排斥的 CRI 在与任一 B 细胞亚群的关联中是分离的。一种 CRI 专门在由 CD5+ B 细胞建立的细胞系中表达，而另一种则与由 CD5- B 细胞建立的细胞系相关。
Human B lymphocytes expressing the CD5 surface antigen (CD5+ B cells) constitute a subset capable of producing polyspecific antibodies recognizing a variety of self antigens. The repertoire of antibodies produced by CD5+ and CD5- B cells is different. However, it is not yet established whether this distribution is reflected in different immunoglobulin variable region gene (IgV) use. Rearrangement of heavy chain IgV (IgVH) genes represents one of the first identifiable stages in the maturation of B cells, and occurs in a developmentally ordered fashion. The repertoire of IgVH gene expression is highly restricted during fetal life but diversifies progressively after birth. A high frequency of VH gene use from the relatively small VHIV gene family has previously been demonstrated in human fetal liver B cells. In the present study, 102 B cell lines established by Epstein-Barr Virus- transformation of separated CD5+ and CD5- cord blood B cells, were examined for the frequency of IgV expression using monoclonal antibodies to cross-reactive idiotypes (CRI). The results demonstrate a relatively high frequency of VHIV gene use (30%) in B cells from cord blood. Furthermore, two mutually exclusive CRI associated with distinct subgroups of the VHIV family are segregated in their association with either subset of B cells. One CRI is exclusively expressed in lines established from CD5+ B cells while the other is associated with lines established from CD5- B cells.