Erythrocyte membrane phospholipid composition as a biomarker of dietary fat
Erythrocyte membrane phospholipid composition as a biomarker of dietary fat
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DOI：
10.1159/000090496
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发表时间：
2006-01-01
影响因子：
3.9
通讯作者：
Muti, P
中科院分区：
文献类型：
--
作者：
Fuhrman, BJ;Barba, M;Muti, P
背景/目的：在一项横断面研究中，我们研究了红细胞膜磷脂脂肪酸组成与膳食脂肪的关系；我们还研究了绝经状态、年龄、体重指数（BMI）和腰臀比（WHR）在生物标志物个体间变异中的作用。方法：研究参与者是204名女性，年龄39 - 65岁，来自ORDET队列，并在乳腺癌研究中选择作为对照。采用毛细管气相色谱法测定膜成分。使用食物频率问卷评估膳食脂肪组成。结果：在绝经前和绝经后的妇女中，亚油酸、油酸和单不饱和脂肪酸的红细胞膜磷脂水平与相应的饮食措施显著相关（偏相关系数分别为：0.23和0.39；0.45和0.47；0.40和0.48）。在绝经后妇女中，膜多不饱和脂肪酸与相应的饮食指标相关（r = 0.39, p < 0.001）。膜二十碳五烯酸和二十二碳己酸水平与鱼类/贝类摄取量显著相关：绝经前r = 0.21和r = 0.43，绝经后r = 0.41和r = 0.44。年龄、BMI和WHR对膜脂组成有独立的影响。年龄与绝经后妇女δ -6去饱和酶活性相关（r = 0.25, p < 0.05）。在绝经前和绝经后妇女中，BMI与δ -9去饱和酶活性呈负相关（r = -0.29, p = 0.01和r = -0.22, p < 0.01）。绝经前妇女WHR与δ -5去饱和酶活性呈负相关（r = - 024, p < 0.05）。结论：某些特定脂肪酸的红细胞膜水平可以作为这些脂肪酸在膳食脂肪中所占比例的生物标志物。版权所有(c) 2006 S. Karger AG，巴塞尔。
Background/Aims: In a cross-sectional study, we investigated the relationship between erythrocyte membrane phospholipid fatty acid composition and dietary fat; we also investigated roles of menopausal status, age, body mass index (BMI) and waist-to-hip ratio (WHR) in interindividual variation of the biomarker. Methods: Study participants were 204 women, aged 39 - 65 years, drawn from the ORDET cohort and selected as controls in a study of breast cancer. Membrane composition was assessed using capillary gas chromatography. Dietary fat composition was evaluated using a food frequency questionnaire. Results: In pre- and postmenopausal women, erythrocyte membrane phospholipid levels of linoleic acid, oleic acid, and mono-unsaturated fatty acids were significantly associated with corresponding dietary measures (partial correlation coefficients: 0.23 and 0.39; 0.45 and 0.47; 0.40 and 0.48; respectively, in pre- and postmenopausal women). Among postmenopausal women, membrane poly-unsaturated fatty acids were correlated with the corresponding dietary measure (r = 0.39, p < 0.001). Membrane eicosapentanoic and docosahexanoic acid levels were significantly correlated with intake of fish/shell fish : r = 0.21 and r = 0.43 (premenopausal), and r = 0.41 and r = 0.44 (postmenopausal). Age, BMI and WHR had independent effects on membrane lipid composition. Age was associated with delta-6 desaturase activity in postmenopausal women (r = 0.25, p < 0.05). BMI was negatively associated with delta-9 desaturase activity in both pre- and postmenopausal women (r = -0.29, p = 0.01 and r = -0.22, p < 0.01, respectively). WHR was negatively associated with delta-5 desaturase activity in pre- menopausal women (r = - 024, p < 0.05). Conclusions: Erythrocyte membrane levels of some specific fatty acids can be used as biomarkers of these fatty acids as proportions of dietary fat. Copyright (c) 2006 S. Karger AG, Basel.