Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection.
Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection.
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DOI：
10.1016/s0264-410x(00)00389-3
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发表时间：
2001-12
期刊：
影响因子：
5.5
通讯作者：
Y. Nishikawa;N. Inoue;X. Xuan;H. Nagasawa;I. Igarashi;K. Fujisaki;H. Otsuka;T. Mikami
中科院分区：
文献类型：
--
作者：
Y. Nishikawa;N. Inoue;X. Xuan;H. Nagasawa;I. Igarashi;K. Fujisaki;H. Otsuka;T. Mikami
构建了表达犬新孢子虫速殖子表面蛋白NcSAG1或NcSRS2的重组牛痘病毒。这些重组病毒的疫苗接种可以有效地保护小鼠模型系统中的寄生虫入侵。 NcSRS2的疫苗效力高于NcSAG1。本研究表明，高水平的寄生虫 IgG1 抗体生产对于感染早期清除寄生虫非常重要，而 T 细胞反应对于感染后期防止细胞内感染起着至关重要的作用。重组痘苗病毒可用作天然宿主中对抗犬新孢子虫感染的疫苗。
The recombinant vaccinia viruses expressing the surface protein of Neospora caninum tachyzoite, NcSAG1 or NcSRS2, were constructed. The vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. The vaccine efficacy of NcSRS2 was higher than that of NcSAG1. The present study indicated that a high level of IgG1 Ab production to parasite is important for clearance of parasite at the early stage of infection and that T cell response has a crucial role for protection against the intracellular infection at the late stage of infection. The recombinant vaccinia viruses might be applicable as vaccine against N. caninum infection in a natural host.