The genome of the CTG(Ser1) yeast S cheffersomyces stipitis is plastic
The genome of the CTG(Ser1) yeast S cheffersomyces stipitis is plastic
复制标题
CTG(Ser1) 酵母 Stipfersomyces stipitis 的基因组是塑料的
DOI：
10.1101/2021.02.15.431239
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Estevez S
中科院分区：
文献类型：
--
作者：
Estevez S
微生物需要适应环境变化，基因组可塑性可以通过增加遗传多样性来快速适应恶劣环境。在这里，我们调查基因组可塑性的CTG（丝氨酸1）酵母Schillosomiastipitis，一种具有巨大潜力的第二代生物燃料生产的有机体。我们证明了S.茎基是一个可塑性基因组，具柄离体植物的基因组具有不同的染色体组织。实时进化实验表明，S.在体外进化实验中，随着广泛的基因组变化和适应性益处的检测，树干基因组可塑性是常见的和快速的。混合MinION Nanopore和Illumina基因组测序将反转录转座子鉴定为基因组多样性的主要驱动因素。事实上，反转录转座子的数量和位置在不同的S中是不同的。基因组的柄状分离物和富含反转录转座子的区域是染色体重排的位点。我们的研究结果为CTG（Ser1）酵母进化枝的适应策略提供了重要的见解，并对第二代生物燃料的开发具有重要意义。这些数据强调了基因组可塑性是可持续发展的一个重要因素。第二代生物燃料生产的平台。
Microorganisms need to adapt to environmental changes, and genome plasticity can lead to rapid adaptation to hostile environments by increasing genetic diversity. Here, we investigate genome plasticity in the CTG(Ser1) yeastScheffersomyces stipitis, an organism with an enormous potential for second-generation biofuel production. We demonstrate thatS. stipitishas an intrinsically plastic genome and that differentS. stipitisisolates have genomes with distinct chromosome organisation. Real-time evolution experiments show thatS. stipitisgenome plasticity is common and rapid as extensive genomic changes with fitness benefits are detected followingin vitroevolution experiments. Hybrid MinION Nanopore and Illumina genome sequencing identifies retrotransposons as major drivers of genome diversity. Indeed, the number and position of retrotransposons is different in differentS. stipitisisolates, and retrotransposon-rich regions of the genome are sites of chromosome rearrangements. Our findings provide important insights into the adaptation strategies of the CTG (Ser1) yeast clade and have critical implications in the development of second-generation biofuels. These data highlight that genome plasticity is an essential factor to be considered for the development of sustainableS. stipitisplatforms for second-generation biofuels production.