Proinflammatory fecal mRNA and childhood bacterial enteric infections
Proinflammatory fecal mRNA and childhood bacterial enteric infections
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DOI：
10.4161/gmic.1.4.13004
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发表时间：
2010-01-01
期刊：
影响因子：
12.2
通讯作者：
Tarr, Phillip, I
中科院分区：
文献类型：
--
作者：
Bennett, William E., Jr.;Gonzalez-Rivera, Rosbel;Tarr, Phillip, I
引言：评估人类样本中的特定mRNA有助于表征疾病。然而，人类粪便中的mRNA研究不足。结果：与对照组相比，感染粪便显示IL-1 β、IL-8和钙卫蛋白的转录增加。mRNA和蛋白质浓度与IL-8相关，但与钙卫蛋白无关。讨论内容：粪便mRNA定量提供了一种潜在有用的、非侵入性的方法来评估胃肠道炎症，并且可能比EIA更敏感。方法：从46例空肠弯曲菌、大肠埃希菌O 157：H7、沙门氏菌感染的儿童粪便中提取RNA。或宋内志贺菌和26名对照，并使用qRT-PCR比较各组之间IL-1 β、IL-8、骨保护素和钙卫蛋白mRNA的比例。我们通过酶免疫测定法测定了同源标本中钙卫蛋白、IL-8和骨保护素的浓度。
Introduction: Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied. Results: Compared to controls, infected stools showed increased transcripts of IL-1 beta, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin. Discussion: Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA. Methods: We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL-1 beta, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.