Genetic diversity in Arabidopsis thaliana L. Heynh. investigated by cleaved amplified polymorphic sequence (CAPS) and inter-simple sequence repeat (ISSR) markers
Genetic diversity in Arabidopsis thaliana L. Heynh. investigated by cleaved amplified polymorphic sequence (CAPS) and inter-simple sequence repeat (ISSR) markers
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DOI：
10.1046/j.0962-1083.2002.01466.x
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发表时间：
2002-03-01
影响因子：
4.9
通讯作者：
Lübberstedt, T
中科院分区：
文献类型：
--
作者：
Barth, S;Melchinger, AE;Lübberstedt, T
本研究利用形态特征和两个基于聚合酶链式反应(PCR)的标记系统：裂解扩增多态序列(CAPS)和简单序列间重复序列(ISSR)，研究了欧亚大陆、北非和北美的37份拟南芥种质资源的遗传多样性。根据CAPS数据计算的遗传相似性进行的聚类分析，大致根据其地理来源对材料进行了分组：一大类包括来自西欧、北欧和南欧以及北非的材料，第二类包括东欧和亚洲大陆的材料。北美种质被散布在这些群中。与CAPS分析相反，从ISSR数据获得的树状图不能反映材料的地理起源，计算的遗传距离与CAPS结果不匹配。这可能是由于ISSR标记的基因组分布不均匀，通过在拟南芥基因组DNA序列文件中对ISSR结合位点的数据库搜索证实了这一点，或者是由于ISSR的不同进化模式。我们推荐CAPS标记用于拟南芥的多样性分析，因为仔细选择标记可以确定整个基因组的均匀代表性。
In this study, we investigated genetic diversity among 37 accessions in Arabidopsis thaliana from Eurasia, North Africa and North America using morphological traits and two polymerase chain reaction (PCR)-based marker systems: cleaved amplified polymorphic sequences (CAPS) and inter-simple sequence repeats (ISSR). Cluster analysis based on genetic similarities calculated from CAPS data grouped the accessions roughly according to their geographical origin: one large group contained accessions from Western, Northern and Southern Europe as well as North Africa, a second group consisted of Eastern European and Asian continental accessions. North American accessions were interspersed into these groups. Contrary to the CAPS analysis, the dendrogram obtained from the ISSR data did not reflect the geographical origin of the accessions, and the calculated genetic distances did not match the CAPS results. This could be attributable to an uneven genomic distribution of ISSR markers as substantiated by a database search for ISSR binding sites in A. thaliana genomic DNA sequence files, or to the ISSR's different mode of evolution. We recommend CAPS markers for diversity analysis in A. thaliana because a careful selection of markers can ascertain an even representation of the entire genome.