Complete replication of hepatitis C virus in cell culture
Complete replication of hepatitis C virus in cell culture
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DOI：
10.1126/science.1114016
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发表时间：
2005-07-22
期刊：
影响因子：
56.9
通讯作者：
Rice, CM
中科院分区：
文献类型：
--
作者：
Lindenbach, BD;Evans, MJ;Rice, CM
丙型肝炎病毒生命周期的许多方面都没有在细胞培养中复制，这减缓了对这一重要人类病原体的研究进展。在这里，我们描述了一个全长的丙型肝炎病毒基因组，它可以复制并产生在细胞培养中具有感染性的病毒颗粒(HCVcc)。HCVcc的复制很强劲，在48小时内每毫升产生近10(5)个感染单位。病毒颗粒可过滤，并用抗病毒糖蛋白E2的单抗中和。病毒的进入依赖于假定的丙型肝炎病毒受体CD81的细胞表达。HCVcc复制被干扰素-α和几种丙型肝炎病毒特异性抗病毒化合物抑制，这表明这个体外系统将有助于寻找改进的抗病毒药物。
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this in vitro system will aid in the search for improved antivirals.