Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation
Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation
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DOI：
10.4236/abb.2010.13029
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发表时间：
2010-08
期刊：
影响因子：
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通讯作者：
I. Stoilova;A. Krastanov;V. Stanchev
中科院分区：
文献类型：
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作者：
I. Stoilova;A. Krastanov;V. Stanchev
一种低成本的生产漆酶的工艺对于可持续的酶法废水处理是必要的。因此，有必要建立一种简单、低成本的漆酶生产工艺。本文研究了用固体底物发酵法从色曲曲霉中提取粗漆酶的性质。研究了该酶在染料脱色中的应用。所研究培养的粗漆酶在45℃时具有最大活性。该酶在40- 47℃和pH为4.5的温度范围内保持了90%以上的活性。测定了粗酶的动力学常数。在KCl、NaCl、CaCl2、MnSO4和MgSO4的存在下，漆酶表现出很高的稳定性，在4个月的孵育后，其初始活性仍保持在50%以上。在CuCl2、FeCl2、FeCl3和NaN3存在的情况下，酶活性在孵育30分钟后完全丧失。靛蓝胭脂红（4 h）、雷马唑亮蓝r （6 h）、橙II - (48 h)和刚果红（13 d）对所研究的粗漆酶100%脱色。
A low-cost process for the production of laccases is necessary for a sustainable enzymatic wastewater treatment. Therefore, it is necessary to establish an easy and low-cost procedure for the production of laccase. In the present study the properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation is investigated. The application of the enzyme for dye decolorization is also studied. Crude laccase from the studied culture established maximal activity at 45oC. The enzyme retained over 90% of its activity in the temperature range 40- 47oC and pH 4.5. The kinetic constants of the crude enzyme was also determined. In the presence of KCl, NaCl, CaCl2, MnSO4 and MgSO4, laccase demonstra- ted high stability—over 50% of its initial activity was still retained after 4-month incubation. Complete loss of enzymatic activity was observed in the presence of CuCl2, FeCl2, FeCl3 and NaN3 after 30 min of incubation. 100% decolorization by investigated crude laccase was completed in the case of Indigo Carmine for 4 h, Remazol Brilliant Blue R—for 6 h, Orange II— for 48 h and Congo Red—for 13 d.