Endometriosis is associated with an altered profile of intestinal microflora in female rhesus monkeys
Endometriosis is associated with an altered profile of intestinal microflora in female rhesus monkeys
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DOI：
10.1093/humrep/17.7.1704
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发表时间：
2002-07-01
影响因子：
6.1
通讯作者：
Coe, CL
中科院分区：
文献类型：
--
作者：
Bailey, MT;Coe, CL
背景技术背景：肠道微生物菌群提供了对肠道病原体的强大防御，并且可能在影响肠道的炎症条件下发生改变，例如子宫内膜异位症。因此，将患有子宫内膜异位症的恒河猴的肠道细菌脱落与年龄匹配的健康对照进行比较。第二项研究评估了雌性猴子肠道炎症的患病率，以确定子宫内膜异位症是否与肠道炎症的可能性增加有关。方法：使用差异性和选择性琼脂对患有或不患有子宫内膜异位症的雌性猴的总需氧菌和革兰氏阴性厌氧菌以及乳酸杆菌进行计数。此外，在尸检报告的回顾性分析中确定了有或无子宫内膜异位症的猴子中肠道炎症的患病率。结果：患有子宫内膜异位症的猴子具有显著不同的脱落菌群。子宫内膜异位症与乳酸杆菌浓度较低和革兰阴性菌浓度较高有关。此外，与健康对照组相比，患有子宫内膜异位症的猴子中肠道炎症的患病率更高。结论：子宫内膜异位症与恒河猴肠道菌群的改变有关。虽然子宫内膜异位症和微生物区系的确切机制尚不清楚，但微生物区系可能受到子宫内膜异位症相关肠道炎症的影响。
BACKGROUND: The intestinal microflora provide a strong defence against intestinal pathogens, and may be altered in inflammatory conditions that impact the gut, such as endometriosis. Therefore, intestinal bacteria shed from rhesus monkeys with endometriosis were compared with age-matched healthy controls. A second study assessed the prevalence of intestinal inflammation in female monkeys to determine whether endometriosis is associated with an increased likelihood of intestinal inflammation. METHODS: Differential and selective agars were used to enumerate total and Gram-negative aerobic and facultatively anaerobic bacteria, as well as Lactobacilli, from female monkeys with or without endometriosis. In addition, the prevalence of intestinal inflammation in monkeys with or without endometriosis was determined in a retrospective analysis of necropsy reports. RESULTS: Monkeys with endometriosis had a significantly different profile of shed microflora. Endometriosis was associated with lower Lactobacilli concentrations and higher Gram-negative bacteria concentrations. Moreover, there was a higher prevalence of intestinal inflammation in monkeys with endometriosis in comparison to healthy controls. CONCLUSIONS: Endometriosis is associated with an altered profile of intestinal microflora in rhesus monkeys. Although the exact mechanisms linking endometriosis and the microflora are unknown, it is possible that the microflora were affected by endometriosis-associated intestinal inflammation.