Rotational-echo double resonance characterization of the effects of vancomycin on cell wall synthesis in Staphylococcus aureus
Rotational-echo double resonance characterization of the effects of vancomycin on cell wall synthesis in Staphylococcus aureus
复制标题
DOI：
10.1021/bi0202326
复制
发表时间：
2002-10-29
期刊：
影响因子：
2.9
通讯作者：
Schaefer, J
中科院分区：
文献类型：
--
作者：
Cegelski, L;Kim, SJ;Schaefer, J
用D-[1-C-13]丙氨酸和[N-15]甘氨酸或[1-C-13]甘氨酸和L-[ε-N-15]赖氨酸标记金黄色葡萄球菌完整细胞，进行交叉极化魔角旋转和旋转回波双共振C-13和N-15 NMR实验。在静止或指数生长条件下收获细胞，后者在添加和不添加万古霉素的培养基中。这些实验的结果允许原位测定肽聚糖交联（共价连接至五甘氨酰桥的肽-茎D-丙氨酸的数量）和桥连接（共价连接至五甘氨酰桥的肽-茎赖氨酸的数量）的相对浓度。在万古霉素存在下，交联的浓度保持恒定，而桥连接的数量减少。这些变化表明万古霉素（在治疗水平）中断了S.金黄色葡萄球菌通过干扰转糖基化。
Cross-polarization magic-angle spinning and rotational-echo double resonance C-13 and N-15 NMR experiments have been performed on intact cells of Staphylococcus aureus labeled with D-[1-C-13]alanine and [N-15]glycine or with [1-C-13]glycine and L-[epsilon-N-15]lysine. The cells were harvested during stationary or exponential growth conditions, the latter in media with and without the addition of vancomycin. The results of these experiments allowed the in situ determination of the relative concentrations of peptidoglycan cross-links (the number of peptide-stem D-alanines covalently linked to a pentaglycyl bridge) and bridge-links (the number of peptide-stem lysines covalently linked to a pentaglycyl bridge). The concentration of cross-links remained constant in the presence of vancomycin, whereas the number of bridge-links decreased. These changes suggest that vancomycin (at therapeutic levels) interrupts peptidoglycan synthesis in S. aureus by interference with transglycosylation.