High levels of virus replication and an intense inflammatory response contribute to the severe pathology in lymphoid tissues caused by Newcastle disease virus genotype VIId
High levels of virus replication and an intense inflammatory response contribute to the severe pathology in lymphoid tissues caused by Newcastle disease virus genotype VIId
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DOI：
10.1007/s00705-014-2301-2
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发表时间：
2015-03-01
影响因子：
2.7
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
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作者：
Hu, Zenglei;Hu, Jiao;Liu, Xiufan
与其他强毒株相比，新城疫病毒 (NDV) 基因型 VIId 的某些毒株会导致淋巴器官更严重的组织损伤。在这项研究中，我们的目的是确定基因 VII 型病毒这种独特病理表现的机制。对感染两种基因型 VIId NDV 毒株（JS5/05 和 JS3/05）、基因型 IX NDV F48E8 和基因型 IV NDV Herts/33 的鸡的淋巴组织的病理学、病毒复制和先天免疫应答进行了比较。组织病理学检查显示，JS5/05和JS3/05在脾脏和胸腺中产生了更严重的病变，但这四种强毒株在法氏囊中引起了相当轻微的病变。此外，JS3/05和JS5/05在淋巴器官中的复制水平显着高于F48E8和Herts/33。对感染 JS5/05 或 Herts/33 的鸡的脾脏进行的微阵列分析表明，JS5/05 通过增加激活基因的数量和表达水平而引发更强烈的炎症反应。此外，细胞因子基因表达谱显示，与 F48E8 和 Herts/33 相比，JS5/05 和 JS3/05 在淋巴组织中诱导更强的细胞因子反应。综上所述，我们的结果表明，由基因型 VIId NDV 毒株引起的免疫器官的严重病理与高水平的病毒复制和强烈的炎症反应有关。
Some strains of Newcastle disease virus (NDV) genotype VIId cause more-severe tissue damage in lymphoid organs compared to other virulent strains. In this study, we aim to define the mechanism of this distinct pathological manifestation of genotype VII viruses. Pathology, virus replication, and the innate immune response in lymphoid tissues of chickens infected with two genotype VIId NDV strains (JS5/05 and JS3/05), genotype IX NDV F48E8 and genotype IV NDV Herts/33, were compared. Histopathologic examination showed that JS5/05 and JS3/05 produced more-severe lesions in the spleen and thymus, but these four virulent strains caused comparable mild lesions in the bursa. In addition, JS3/05 and JS5/05 replicated at significantly higher levels in the lymphatic organs than F48E8 and Herts/33. A microarray assay performed on the spleens of chickens infected with JS5/05 or Herts/33 revealed that JS5/05 elicited a more potent inflammatory response by increasing the number and expression levels of activated genes. Moreover, cytokine gene expression profiling showed that JS5/05 and JS3/05 induced a stronger cytokine response in lymphoid tissues compared to F48E8 and Herts/33. Taken together, our results indicate that the severe pathology in immune organs caused by genotype VIId NDV strains is associated with high levels of virus replication and an intense inflammatory response.