FURTHER PURIFICATION OF A POLYPEPTIDE DEMONSTRATING ENTEROGASTRONE ACTIVITY
FURTHER PURIFICATION OF A POLYPEPTIDE DEMONSTRATING ENTEROGASTRONE ACTIVITY
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DOI：
10.1113/jphysiol.1970.sp009155
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发表时间：
1970-01-01
影响因子：
5.5
通讯作者：
PEDERSON, RA
中科院分区：
文献类型：
--
作者：
BROWN, JC;MUTT, V;PEDERSON, RA
1.描述了进一步纯化具有有效肠抑胃素活性而无CCK-PZ效应的多肽。2.当静脉内给予1.0 μg/kg.hr剂量时，该物质具有抑制作用。氨基酸分析表明，脯氨酸的情况下，谷氨酰胺和赖氨酸的优势超过精氨酸的含量高。胰蛋白酶降解破坏多肽的抑制作用。在确定多肽的生理状态之前，必须进行进一步的研究。
1. The further purification of a polypeptide having potent enterogastrone activity, without CCK—PZ effects, is described. 2. The material was inhibitory when doses of 1·0 μg/kg.hr were administered intravenously. Amino acid analyses demonstrated the absence of proline, a high content of glutamine and a preponderance of lysine over arginine. Tryptic degradation destroys the inhibitory effect of the polypeptide. Further studies must be performed before the physiological status of the polypeptide can be ascertained.