JUNCTION POTENTIALS, ELECTRODE STANDARD POTENTIALS, AND OTHER PROBLEMS IN INTERPRETING ELECTRICAL PROPERTIES OF MEMBRANES
JUNCTION POTENTIALS, ELECTRODE STANDARD POTENTIALS, AND OTHER PROBLEMS IN INTERPRETING ELECTRICAL PROPERTIES OF MEMBRANES
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DOI：
10.1007/bf01868023
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发表时间：
1970-01-01
影响因子：
2.4
通讯作者：
DIAMOND, JM
中科院分区：
文献类型：
--
作者：
BARRY, PH;DIAMOND, JM
有人建议，电动耦合可能涉及的机制，动作电位，因此，应该有随之而来的体积流量和压力变化与这种兴奋。在以前的论文中，这样的测量报告在细胞ofChara australis，从它也是已知的，在兴奋过程中有一个增加的KCl渗透性和外流的KCl。在本文中，一些理论分析已被认为是和发展有关的这种测量和时间依赖性的关系表观测量体积流量，真实的体积流量和膨压变化的细胞在各种实验情况下。这种体积流主要是通过K+和Cl−离子对水的摩擦耦合来定量解释的，在较小程度上是由于KCl增强了细胞壁-膜界面处的局部渗透流。在激发过程中测得的12×10− 3到28×10−3atm的压力变化也被正确地预测为从细胞中流出的体积的结果，该细胞表现为液压泄漏的弹性圆柱体，因此压力下降。然后，这些结论表明，体积流量和压力变化测量的膜渗透性的变化的附带后果，并不一定意味着任何电动机制的动作电位本身。
It has been suggested that electro-kinetic coupling may be involved in the mechanism of the action potential and that there should therefore be both consequent volume flows and pressure changes associated with such excitation. In a previous paper, such measurements were reported in cells ofChara australis, from which it is also known that during excitation there is an increase in KCl permeability and an efflux of KCl. In this paper, a number of theoretical analyses have been considered and developed pertaining to such measurements and the time-dependent relationships between apparent measured volume flows, true volume flows and turgor pressure changes in cells in various experimental situations. Such volume flows are quantitatively explained primarily from the frictional coupling of water by both K+and Cl−ions and to a lesser extent by the local osmotic flow owing to KCl enhancement at the wall-membrane interface of the cell. The measured pressure changes of 12×10−3to 28×10−3atm during excitation are also correctly predicted as the result of such a volume outflow from the cell which behaves as a hydraulically leaky elastic cylinder and thereby drops in pressure. These conclusions then indicate that the volume flows and pressure changes measured are the incidental consequences of a change in membrane permeability and do not necessarily imply any electro-kinetic mechanism for the action potential itself.