Five-Antigen Fluorescent Bead-Based Assay for Diagnosis of Lyme Disease
Five-Antigen Fluorescent Bead-Based Assay for Diagnosis of Lyme Disease
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DOI：
10.1128/cvi.00685-15
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发表时间：
2016-04-01
影响因子：
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通讯作者：
Tardo, Amanda C.
中科院分区：
文献类型：
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作者：
Embers, Monica E.;Hasenkampf, Nicole R.;Tardo, Amanda C.
诊断莱姆病的系统性困难任务可以通过敏感和特异性的实验室检查来简化。目前推荐的两级血清学试验具有高度特异性，但灵敏度福尔斯不足，尤其是在急性期早期。我们先前检查了从伯氏疏螺旋体感染的恒河猴中连续收集的血清样品，并定义了可用于检测人类疾病所有阶段感染的抗原组合。五个B。将由OspC、OspA、DbpA、OppA 2和C6肽组成的伯氏螺旋体抗原组合到基于荧光细胞计数珠的测定中，用于检测B。burgdorferi抗原特异性IgG抗体。来自莱姆病患者和对照的样品用于确定该测定的诊断价值。使用该样本集，我们发现我们的五抗原多重IgG检测具有更高的灵敏度（79.5%）比酶免疫测定（EIA）（76.1%），两级测试（61.4%），和C6肽酶联免疫吸附试验（ELISA）（77.2%），同时保持特异性超过90%。当将IgM检测添加到基于珠的测定中时，灵敏度提高到91%，但代价是特异性降低（78%）。这些结果表明，在我们的多重检测抗原的合理组合，可以提供一个改进的莱姆病血清学诊断试验。
The systematically difficult task of diagnosing Lyme disease can be simplified by sensitive and specific laboratory tests. The currently recommended two-tier test for serology is highly specific but falls short in sensitivity, especially in the early acute phase. We previously examined serially collected serum samples from Borrelia burgdorferi-infected rhesus macaques and defined a combination of antigens that could be utilized for detection of infection at all phases of disease in humans. The five B. burgdorferi antigens, consisting of OspC, OspA, DbpA, OppA2, and the C6 peptide, were combined into a fluorescent cytometric bead-based assay for the detection of B. burgdorferi antigen-specific IgG antibodies. Samples from Lyme disease patients and controls were used to determine the diagnostic value of this assay. Using this sample set, we found that our five-antigen multiplex IgG assay exhibited higher sensitivity (79.5%) than the enzyme immunoassay (EIA) (76.1%), the two-tier test (61.4%), and the C6 peptide enzyme-linked immunosorbent assay (ELISA) (77.2%) while maintaining specificity over 90%. When detection of IgM was added to the bead-based assay, the sensitivity improved to 91%, but at a cost of reduced specificity (78%). These results indicate that the rational combination of antigens in our multiplex assay may offer an improved serodiagnostic test for Lyme disease.