A supramolecular biosensor for monitoring lysosomal DNase activity in vivo and identifying tumor cells by responding to G-quadruplex hydrolysis
A supramolecular biosensor for monitoring lysosomal DNase activity in vivo and identifying tumor cells by responding to G-quadruplex hydrolysis
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DOI：
10.1016/j.snb.2023.133664
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发表时间：
2023-06
期刊：
影响因子：
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通讯作者：
Dawei Yang;Hongxia Sun;Ranran Sun;Yan Liu;Hong Zhang;Li-Xia Wang;Liyun Yao;Yalin Tang
中科院分区：
文献类型：
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作者：
Dawei Yang;Hongxia Sun;Ranran Sun;Yan Liu;Hong Zhang;Li-Xia Wang;Liyun Yao;Yalin Tang
溶酶体DNA酶是维持细胞DNA合成和代谢稳态所必需的，其异常活性导致多种疾病，如癌症和免疫异常。在此，一个名为PTC-G4的生物传感器已被构建用于检测溶液和活生物体中的溶酶体DNA酶活性。该生物传感器由花青染料（PTC）超分子和外源性G-四链体（G4）DNA组成，这两者都高度靶向于活细胞中的溶酶体，而PTC对G4结构具有高度特异性。通过监测PTC单体和聚集体响应于G4水解的双通道荧光来评价溶酶体DNA酶活性。PTC-G4不仅可以作为体内监测溶酶体DNA酶的工具，而且可以区分肿瘤细胞和正常细胞的差异，使其在肿瘤筛查或辅助诊断方面具有前景。
Lysosomal DNase is essential for maintaining cellular DNA synthesis and metabolic homeostasis, and their abnormal activity leads to a variety of diseases such as cancer and immune abnormalities. Herein, a biosensor named PTC-G4 has been constructed to detect lysosomal DNase activity in solution and living organisms. This biosensor consists of a cyanine dye (PTC) supramolecule and an exogenous G-quadruplex (G4) DNA, both of which are highly targeted to lysosomes in live cells, while PTC is highly specific for the G4 structure. Lysosomal DNase activity was evaluated by monitoring the double-channel fluorescence of PTC monomers and aggregates in response to G4 hydrolysis. PTC-G4 can not only be used as a tool for monitoring lysosomal DNase in vivo, but also can distinguish the difference between tumor cells and normal cells, making it promising in tumor screening or auxiliary diagnosis.