Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata
Crystallization and preliminary crystallographic study of oligomers of the haemolytic lectin CEL-III from the sea cucumber Cucumaria echinata
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海参溶血凝集素 CEL-III 低聚物的结晶和初步晶体学研究
DOI：
10.1107/s1744309113004065
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
and Tomomitsu Hatakeyama
中科院分区：
文献类型：
--
作者：
Hideaki Unno;Keigo Hisamatsu;Tomonao Nagao;Yuki Tateya;Naoki Matsumoto;Shuichiro Goda;and Tomomitsu Hatakeyama
CEL-III是从海洋无脊椎动物刺瓜（Cucumaria echinata）中分离的一种钙依赖性溶血凝集素。这种凝集素结合到细胞表面上的含Gal/GalNAc的碳水化合物链上，并且在构象变化后，寡聚化以在细胞膜中形成离子渗透孔。CEL-III还形成可溶性低聚物，类似于在高pH和高盐溶液中结合特定碳水化合物时在细胞膜中形成的那些。将这些可溶性和膜CEL-III低聚物结晶并收集X射线衍射数据。晶体的可溶性低聚物和膜低聚物衍射X射线，分别为3.3和4.2毫米的分辨率，使用同步辐射和前者被发现属于空间群C2。 可溶性低聚物晶体的自旋转功能分析表明，它可能是由七聚体CEL-III。
CEL-III is a Ca2+-dependent haemolytic lectin isolated from the marine invertebrate Cucumaria echinata. This lectin binds to Gal/GalNAc-containing carbohydrate chains on the cell surface and, after conformational changes, oligomerizes to form ion-permeable pores in cell membranes. CEL-III also forms soluble oligomers similar to those formed in cell membranes upon binding of specific carbohydrates in high-pH and high-salt solutions. These soluble and membrane CEL-III oligomers were crystallized and X-ray diffraction data were collected. Crystals of soluble oligomers and membrane oligomers diffracted X-rays to 3.3 and 4.2 Å resolution, respectively, using synchrotron radiation and the former was found to belong to space group C2. Self-rotation functional analysis of the soluble oligomer crystal suggested that it might be composed of heptameric CEL-III.