Population Genetic Analysis of Propionibacterium acnes Identifies a Subpopulation and Epidemic Clones Associated with Acne
Population Genetic Analysis of Propionibacterium acnes Identifies a Subpopulation and Epidemic Clones Associated with Acne
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DOI：
10.1371/journal.pone.0012277
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发表时间：
2010-08-19
期刊：
影响因子：
3.7
通讯作者：
Kilian, Mogens
中科院分区：
文献类型：
--
作者：
Lomholt, Hans B.;Kilian, Mogens
痤疮丙酸杆菌在痤疮发病机制中的参与是有争议的，主要是由于其作为健康皮肤的居民的优势。这项研究验证了这样的假设：该物种的特定进化谱系与痤疮有关，而其他谱系则与健康状况相符。基于9个管家基因对210株痤疮丙酸杆菌进行了系统发育重建，这些分离株来自特征良好的痤疮患者、各种机会性感染和健康携带者。虽然重组的证据进行了观察，结果表明，基本上克隆的人口结构与等位基因变异的毒力基因tly和camp5，脉冲场凝胶电泳（PFGE）和生物型，并表达推定的毒力因子。一个意想不到的地理和时间广泛传播的一些克隆被证明。人群包括三个主要部门，其中之一，包括流行性克隆，与中度至重度痤疮密切相关，而其他人则与健康和机会性感染有关。这种二分法与先前观察到的体外炎症诱导特性的差异相关。比较代表痤疮和健康相关克隆的五个基因组，发现多个簇和菌株特异性基因，表明生态偏好的重大差异，并重新定义了疾病相关毒力因子的谱。研究结果表明，痤疮丙酸杆菌的特定克隆在痤疮中起病因作用，而其他克隆则与健康有关。
The involvement of Propionibacterium acnes in the pathogenesis of acne is controversial, mainly owing to its dominance as an inhabitant of healthy skin. This study tested the hypothesis that specific evolutionary lineages of the species are associated with acne while others are compatible with health. Phylogenetic reconstruction based on nine housekeeping genes was performed on 210 isolates of P. acnes from well-characterized patients with acne, various opportunistic infections, and from healthy carriers. Although evidence of recombination was observed, the results showed a basically clonal population structure correlated with allelic variation in the virulence genes tly and camp5, with pulsed field gel electrophoresis (PFGE)- and biotype, and with expressed putative virulence factors. An unexpected geographically and temporal widespread dissemination of some clones was demonstrated. The population comprised three major divisions, one of which, including an epidemic clone, was strongly associated with moderate to severe acne while others were associated with health and opportunistic infections. This dichotomy correlated with previously observed differences in in vitro inflammation-inducing properties. Comparison of five genomes representing acne-and health-associated clones revealed multiple both cluster-and strain-specific genes that suggest major differences in ecological preferences and redefines the spectrum of disease-associated virulence factors. The results of the study indicate that particular clones of P. acnes play an etiologic role in acne while others are associated with health.