A GABA ANTAGONIST, BICUCULLINE, EXERTS ITS UNCOUPLING ACTION ON EXTERNAL HORIZONTAL CELLS THROUGH DOPAMINE CELLS IN CARP RETINA
A GABA ANTAGONIST, BICUCULLINE, EXERTS ITS UNCOUPLING ACTION ON EXTERNAL HORIZONTAL CELLS THROUGH DOPAMINE CELLS IN CARP RETINA
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DOI：
10.1016/0304-3940(83)90441-x
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发表时间：
1983-01-01
影响因子：
2.5
通讯作者：
KATO, S
中科院分区：
文献类型：
--
作者：
NEGISHI, K;TERANISHI, T;KATO, S
在离体的鲤鱼视网膜中，外水平细胞（C。carpio）进行细胞内记录并用荧光染料荧光黄（LY）标记。这些细胞通过间隙连接电耦合，使得注入的染料通常扩散到相邻的细胞。将GABA拮抗剂荷包牡丹碱（Bcc，20 μ M）应用于离体视网膜下的玻璃体液，通过增加对中心点的响应幅度和降低对远点的响应幅度，改变了光诱导响应的空间特性。BCC也仅限于将染料注入单个记录细胞。Bcc的这些解偶联作用类似于安非他明（20 μ M）或多巴胺（10-20 μ M）的解偶联作用，并且被多巴胺能阻断剂氟哌啶醇（20-40 μ M）的存在所消除。BCC和安非他明的行动时，没有观察到应用于视网膜剥夺多巴胺能细胞的事先破坏与6-羟基多巴胺。Bcc通过多巴胺能系统间接对视网膜外水平细胞发挥解偶联作用。
External horizontal cells in isolated retinas of the carp (C. carpio) were intracellularly recorded and marked with the fluorescent dye Lucifer Yellow (LY). These cells are electrically coupled via gap junctions, so that the injected dye normally diffused to neighboring cells. A GABA antagonist, bicuculline (Bcc, 20 .mu.M), applied to the vitreous fluid beneath the isolated retina, altered the spatial properties of light-induced responses, by increasing the amplitude of responses to central spots and decreasing that of those to distant spots. Bcc also restricted to injected dye to single recorded cells. These uncoupling actions of Bcc were similar to those of amphetamine (20 .mu.M) or dopamine (10-20 .mu.M) and were abolished by the presence of a dopaminergic blocker, haloperidol (20-40 .mu.M). Both the actions of Bcc and amphetamine were not observed when applied to retinas deprived of dopaminergic cells by prior destruction with 6-hydroxydopamine. Bcc exerts its uncoupling actions on external horizontal cells indirectly through the dopaminergic system in the carp retina.