A modified method for rapid quantification of Chlamydia muridarum using Fluorospot.
A modified method for rapid quantification of Chlamydia muridarum using Fluorospot.
复制标题
使用 Fluorospot 快速定量鼠衣原体的改进方法。
DOI：
10.1016/j.mex.2019.08.005
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发表时间：
2019
期刊：
影响因子：
1.9
通讯作者：
Arulanandam,BernardP
中科院分区：
文献类型：
--
作者：
Keck,Jonathon;Chambers,JamesP;Forsthuber,Thomas;Gupta,Rishein;Arulanandam,BernardP
虽然手工计数衣原体包涵体形成单位是该领域最广泛接受的定量方法，但它既耗时又易受研究者固有偏倚的影响。我们在这里报告一个快速，即，分钟vs.小时，改良的自动化Fluorospot评估方法是线性的（<1200点/孔）。由于Fluorospot计数的组织培养板/孔也可以使用传统的手动计数进行定量，因此新获得的Fluorospot数据可以很容易地与先前建立的手动计数数据进行比较，无需新的参考标准。·在同一组织培养板上使用自动和手动计数方法同时计数衣原体IFU。·快速计数衣原体的方法IFU将时间从数小时缩短到数分钟。
Although manual enumeration ofChlamydiainclusion forming units is the most widely accepted means of quantification in the field, it is both time consuming and subject to inherent investigator bias. We report here a rapid,i.e., minutesvs. hours, modified automated Fluorospot means of assessment that is linear (<1200 dots per well). Because the Fluorospot enumerated tissue culture plate/well can also be quantified using traditional manual counting, newly derived Fluorospot data can easily be compared to previously established manual enumeration data requiring no new reference norms.•Concurrent enumeration of chlamydial IFU using automated and manual methods of counting on same tissue culture plate.•Rapid method of counting chlamydial IFU reducing time from hours to minutes.