The Spatial Context of Tumor-Infiltrating Immune Cells Associates with Improved Ovarian Cancer Survival.
The Spatial Context of Tumor-Infiltrating Immune Cells Associates with Improved Ovarian Cancer Survival.
复制标题
肿瘤浸润免疫细胞的空间环境与卵巢癌患者生存率提高相关。
DOI：
10.1158/1541-7786.mcr-21-0411
复制
发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Wrobel J
中科院分区：
文献类型：
--
作者：
Steinhart B;Jordan KR;Bapat J;Post MD;Brubaker LW;Bitler BG;Wrobel J
卵巢癌是最致命的妇科恶性肿瘤。多组学技术为治疗反应和患者结果的改进预测建模提供了平台。虽然高级别浆液性癌（HGSOC）肿瘤具有免疫原性，并且许多研究已经确定了与免疫细胞浸润的正相关性，但临床试验中的免疫疗法表现出低疗效率。有一个显着的需要，以更好地理解免疫细胞的作用和组成，介导卵巢癌的治疗反应和进展。我们用HGSOC组织微阵列（n=127）进行多重免疫组织化学以表征肿瘤内的免疫细胞组成。在分析肿瘤内T细胞（CD 4/CD 8）、巨噬细胞（CD 68）和B细胞（CD 19）的组成和空间背景后，我们发现B细胞和CD 4 T细胞的存在与总生存率相关。更重要的是，我们观察到肿瘤相关巨噬细胞和B细胞或CD 4 T细胞之间的接近度与总生存率显著相关。
Ovarian cancer is the deadliest gynecological malignancy. Multi-omics techniques have provided a platform for improved predictive modeling of therapy response and patient outcomes. While high-grade serous carcinoma (HGSOC) tumors are immunogenic and numerous studies have defined positive correlation to immune cell infiltration, immunotherapies in clinical trials have exhibited low efficacy rates. There is a significant need to better comprehend the role and composition of immune cells in mediating ovarian cancer therapeutic response and progression. We performed multiplex immunohistochemistry with an HGSOC tissue microarray (n=127) to characterize the immune cell composition within tumors. After analyzing the composition and spatial context of T cells (CD4/CD8), macrophages (CD68), and B cells (CD19) within the tumor, we found that increased B cell and CD4 T cell presence correlated with overall survival. More importantly, we observed that the proximity between tumor-associated macrophages and B cells or CD4 T cells significantly correlated with overall survival.