Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China
Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China
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DOI：
10.1016/j.scitotenv.2019.03.172
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发表时间：
2019-06-15
影响因子：
9.8
通讯作者：
Chen, Lijun
中科院分区：
文献类型：
--
作者：
Chen, Xiaodong;Jiang, Nan;Chen, Lijun
细菌phoD基因编码碱性磷酸单酯酶，该酶在土壤有机磷转化为植物有效无机磷的过程中起重要作用。然而，phoD基因群落，碱性磷酸单酯酶活性，并在土壤中的磷有效性之间的关系知之甚少。在这项研究中，我们调查了碱性磷酸单酯酶活性，phoD基因丰度，和社区结构的影响，植物有效磷使用土壤（0-10，10-20和20-40厘米）从长期的田间试验中，连续玉米（玉米L.）作物连续28年接受不同水平的磷肥投入（30、60 kg P ha（-1）年（-1））。采用定量PCR和高通量测序分析phoD基因丰度和群落组成。碱性磷酸单酯酶活性与土壤速效磷呈负相关，这反映在phoD基因丰度的相应数据上。另一方面，phoD基因α多样性和有效磷之间的正相关性，而phoD基因群落结构的变化与土壤pH值和磷的有效性的变化。假单胞菌相对丰度与土壤磷有效性呈负相关，与碱性磷酸单酯酶活性呈正相关，表明假单胞菌可能在土壤有机磷矿化中发挥重要作用。研究结果表明，长期施用磷肥导致土壤磷素有效性的变化，通过调节phoD基因丰度、多样性以及改变phoD基因群落组成，显著影响碱性磷酸单酯酶活性。(C)2019由Elsevier B. V.出版
The bacterial phoD gene encodes alkaline phosphomonoesterase, an enzyme which plays an important role in the release of plant-available inorganic phosphorus (P) from organic P in soil. However, the relationships between phoD gene community, alkaline phosphomonoesterase activity, and P availability in soil are poorly understood. In this study, we investigated how alkaline phosphomonoesterase activity, phoD gene abundance, and community structure are influenced by plant-available P using soils (0-10, 10-20 and 20-40 cm) from a long-term field trial in which a continuous maize (Zea mays L.) crop had received different levels of P fertilizer inputs (30, 60 kg P ha(-1) year(-1)) for 28 years. Quantitative PCR and high-throughput sequencing were used to analyze phoD gene abundance and community composition. Alkaline phosphomonoesterase enzyme activity was negatively correlated with soil available P, which was reflected in corresponding data for phoD gene abundance. On the other hand, positive correlations were determined between phoD gene alpha-diversity and available P, while shifts in phoD gene community structure were related to changes in soil pH and P availability. The relative abundance of Pseudomonas was negatively correlated with P availability and positively correlated with alkaline phosphomonoesterase activity, suggesting that Pseudomonas may play an important role in soil organic P mineralization. The findings of this study demonstrated that changes of soil P availability as a result of long-term P fertilizer inputs significantly affected alkaline phosphomonoesterase activity by regulating phoD gene abundance, diversity, as well as altering the phoD gene community composition. (C) 2019 Published by Elsevier B.V.