Examination of early interactions between Haemophilus ducreyi and host cells by using cocultured HaCaT keratinocytes and foreskin fibroblasts.
Examination of early interactions between Haemophilus ducreyi and host cells by using cocultured HaCaT keratinocytes and foreskin fibroblasts.
复制标题
使用共培养的 HaCaT 角质形成细胞和包皮成纤维细胞检查杜克雷嗜血杆菌与宿主细胞之间的早期相互作用。
DOI：
10.1128/iai.67.10.5352-5360.1999
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发表时间：
1999
影响因子：
3.1
通讯作者：
Kawula,TH
中科院分区：
文献类型：
--
作者：
Zaretzky,FR;Kawula,TH
Haemophilus ducreyiis the etiologic agent of chancroid, a sexually transmitted genital ulcer disease. Keratinocytes are likely the first cell type encountered byH. ducreyiupon infection of human skin; thus, the interaction betweenH. ducreyiand keratinocytes is probably important for the ability ofH. ducreyito establish infection. We have used the HaCaT keratinocyte cell line grown in monolayers and in cocultures with HS27 fibroblasts to investigateH. ducreyiinteractions with keratinocytes and the host-cell response toH. ducreyiinfection. Using quantitative adherence and gentamicin protection assays, we determined that approximately 13% ofH. ducreyiadhered to HaCaT cell monolayers, while only a small proportion (0.0052%) was intracellular. By transmission electron microscopy, we observed numerousH. ducreyiorganisms adherent to but rarely within HaCaT cells cocultured with fibroblasts. Both liveH. ducreyiand purifiedH. ducreyilipooligosaccharide (LOS) induced significant interleukin 8 (IL-8) expression from HaCaT cell-HS27 cell cocultures. However, the level of IL-8 expression in response to LOS alone was not as pronounced.H. ducreyiLOS was a more potent inducer of IL-8 from cocultures thanEscherichia colilipopolysaccharide (LPS) at the same concentration, suggesting a unique effect ofH. ducreyiLOS on cocultures. Neither liveH. ducreyinor purifiedH. ducreyiLOS orE. coliLPS induced tumor necrosis factor alpha expression from cocultures.H. ducreyiinduced drastically different cytokine profiles from cocultures than from HS27 or HaCaT cells cultured separately. IL-8 expression by skin cells in response toH. ducreyiinfection in vivo may be responsible for the massive influx of polymorphonuclear leukocytes and other inflammatory cells to the site of infection. This influx of inflammatory cells may be partly responsible for the tissue destruction characteristic of chancroid.
