Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements
Elevated Plus Maze Test Combined with Video Tracking Software to Investigate the Anxiolytic Effect of Exogenous Ketogenic Supplements
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DOI：
10.3791/58396
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发表时间：
2019-01-01
影响因子：
1.2
通讯作者：
Kovacs, Zsolt
中科院分区：
文献类型：
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作者：
Ari, Csilla;D'Agostino, Dominic P.;Kovacs, Zsolt
本研究的总体目标是描述高架十字迷宫（高架十字迷宫）测试与视频跟踪软件相结合的方法。该方法的目的是记录各种潜在抗焦虑治疗对实验室啮齿动物模型的影响。该测试基于啮齿动物对受保护的封闭黑暗空间的倾向，以及对开放空间和高度的无条件恐惧，以及它们探索新环境的内在强烈动机。抗焦虑试验是一种广泛使用的行为试验，用于研究给予已知会影响行为的药物的啮齿动物的抗焦虑或致焦虑反应。观察结果表明，通过焦虑测试测量的闭合臂上花费的时间比例降低，开放臂上花费的时间比例增加，进入闭合臂的次数减少，以及进入开放臂的次数增加，这可能反映了焦虑水平降低。使用该方法，在Sprague道利（SPD）大鼠中测试外源性酮补充剂对焦虑相关行为的影响。在进行抗氧化试验之前，将外源性酮补充剂长期喂饲大鼠83天，或亚慢性和急性经口管饲，每天一次，持续7天。在治疗结束时，由盲态观察者使用SMART视频跟踪系统进行行为数据收集。主要研究结果表明，抗焦虑试验是检测酮替芬诱导的抗焦虑作用的有效方法，可以被认为是评估与药物或代谢治疗相关的焦虑行为变化的敏感指标。
The overall goal of this study is to describe the methodology of the elevated plus maze (EPM) test in combination with a video tracking software. The purpose of the method is to document the effect of various potential anxiolytic treatments on laboratory rodent models. The EPM test is based on the rodents' proclivity toward protected, enclosed dark spaces and unconditioned fear of open spaces and heights, and their innate intense motivation to explore novel environments. The EPM test is a widely used behavioral test for investigating the anxiolytic or anxiogenic responses of rodents given drugs that are known to affect behavior. Observation demonstrating a decreased proportion of time spent on closed arms, an increased proportion of time spent on open arms, a reduced number of entries to closed arms, and an elevated number of entries to open arms measured by the EPM test may reflect reduced anxiety levels. Using this method, the effect of exogenous ketone supplements on anxiety-related behavior is tested in Sprague Dawley (SPD) rats. Exogenous ketone supplements are chronically fed to the rats for 83 days or subchronically and acutely orally gavaged, daily for 7 days, before conducting the EPM test. Behavioral data collection is performed using the SMART video tracking system by a blinded observer at the end of the treatments. The main findings indicate that the EPM test is an effective method to detect the ketone supplement-induced anxiolytic effect and can be considered a sensitive measure to assess changes in anxiety behavior associated with drug- or metabolic-based therapies.