Photoacoustic lymphangiography
Photoacoustic lymphangiography
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DOI：
10.1002/jso.25575
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发表时间：
2020-01-01
影响因子：
2.5
通讯作者：
Kishi, Kazuo
中科院分区：
文献类型：
--
作者：
Kajita, Hiroki;Oh, Anna;Kishi, Kazuo
背景和目的光声淋巴管造影术是一种基于光声技术的光学成像技术，它可以以高空间分辨率显示淋巴管中血红蛋白或黑色素等光吸收组织成分以及吲哚菁绿色（ICG）等光吸收造影剂的分布。在这份报告中，我们介绍了三维（3D）图像的人体淋巴管获得光声淋巴管造影。方法采用派-05三维光声成像系统。招募了一些健康受试者和水肿患者。为了使肢体的淋巴结构成像，如在荧光淋巴管造影术中一样皮下施用ICG。光声图像通过使用近红外区域波长的激光照射组织来获取。同时，还记录了荧光图像。结果在三维立体图上可观察到直径达0.2mm的淋巴管及其周围的微静脉。在患者组中，经常观察到真皮回流模式为淋巴管的密集互连3D结构。还观察到通过真皮下的收集血管，这在荧光淋巴造影中未观察到。结论光声淋巴管造影可对淋巴管进行详细的观察，从而加深对淋巴管三维结构和生理状态的认识。
Background and objectives Photoacoustic lymphangiography, which is based on photoacoustic technology, is an optical imaging that visualizes the distribution of light absorbing tissue components like hemoglobin or melanin, as well as optical absorption contrast imaging agents like indocyanine green (ICG) in the lymphatic channels, with high spatial resolution. In this report, we introduce the three-dimensional (3D) images of human lymphatic vessels obtained with photoacoustic lymphangiography. Methods We used the 3D photoacoustic visualization system (PAI-05). Some healthy subjects and lymphedema patients were recruited. To image the lymphatic structures of the limbs ICG was administered subcutaneously as in fluorescence lymphangiography. Photoacoustic images were acquired by irradiating the tissue using a laser at wavelengths of near-infrared region. On the same occasion, fluorescence images were also recorded. Results The lymphatic vessels up to the diameter of 0.2 mm could be observed three-dimensionally with the venules around them. In the patient-group, dermal backflow patterns were often observed as dense interconnecting 3D structures of lymphatic vessels. Collecting vessels passing below the dermis were also observed, which were not observed by fluorescence lymphography. Conclusions Photoacoustic lymphangiography provided the detailed observation of each lymphatic vessel, leading to deeper understanding of 3D structures and physiological state of the vessel.